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Studying M1 and M2 States in Adult Microglia

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Microglia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1041))

Abstract

Microglial cell function receives increasing interest. To date, the majority of experiments are performed by using immortalized microglia-like cells or primary microglia prepared from pre- or postnatal rodent brain. As those may not adequately reflect the microglial biology in the adult brain, this protocol advocates a procedure which allows for the isolation, purification, and subsequent analysis of microglial cells. Once isolated, the principal state of activation, M1 or M2, can be determined in adult microglia using fluorescence-activated cell sorting, quantitative PCR, and/or Western blotting. Likewise, adult microglia generated by this protocol can be used for functional analysis through cell cultivation for a limited time.

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Acknowledgement

This work was supported by a grant of the Deutsche Forschungsgemeinschaft to MTH (KFO177, TP8) and of the EU-FP7 program on neuroinflammation (INMIND).

Author information

Authors and Affiliations

  1. University of Bonn, Bonn, Germany

    Sadanand M. Gaikwad

  2. Clinical Neuroscience Unit, Department of Neurology, University of Bonn, Bonn, Germany

    Michael T. Heneka

Authors
  1. Sadanand M. Gaikwad
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  2. Michael T. Heneka
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Editor information

Editors and Affiliations

  1. , Department of Oncology-Pathology, Karolinska Institutet, Solnavägen, Stockholm, SE-171 77, Sweden

    Bertrand Joseph

  2. , Department of Biochemistry, University of Seville, García González 2, Seville, 41012, Spain

    José Luis Venero

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© 2013 Springer Science+Business Media New York

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Gaikwad, S.M., Heneka, M.T. (2013). Studying M1 and M2 States in Adult Microglia. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_18

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  • DOI: https://doi.org/10.1007/978-1-62703-520-0_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-519-4

  • Online ISBN: 978-1-62703-520-0

  • eBook Packages: Springer Protocols

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