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The Golgi–Cox Method

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Neural Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1018))

Abstract

One of the best neurohistologic methods to reveal the cytoarchitecture of the brain and detailed morphology of neurons with unsurpassed clarity has been the Golgi staining. It is based on the principle of metallic impregnation of neurons, allowing visualization in their entirety including cell soma, axons, dendrites, and spines. In this chapter, we describe the Golgi–Cox protocol standardized in our laboratory that can be used to study experimental effects of different genetic manipulations on spatial distribution of neurons, dendrite density, spine number and morphology to elucidate gene functions during development and in adult brain.

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References

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Acknowledgment

This work has been supported by NIH grant RO1EY019012 and PO1HD023315 to R.Z.

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Authors and Affiliations

  1. Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA

    Gitanjali Das & Renping Zhou

  2. Neurotoxicology Laboratory, Department of Pharmacology and Toxicology, Rutgers University, Piscataway, NJ, USA

    Kenneth Reuhl

Authors
  1. Gitanjali Das
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  2. Kenneth Reuhl
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  3. Renping Zhou
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Editor information

Editors and Affiliations

  1. , Department of Chemical Biology, Rutgers University, Frelinghuysen Road 164, Piscataway, 08854, New Jersey, USA

    Renping Zhou

  2. , Inst. Molecular Medicine & Genetics, Georgia Health Sciences University, 15th Street 1120, Augusta, 30912, Georgia, USA

    Lin Mei

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Das, G., Reuhl, K., Zhou, R. (2013). The Golgi–Cox Method. In: Zhou, R., Mei, L. (eds) Neural Development. Methods in Molecular Biology, vol 1018. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-444-9_29

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  • DOI: https://doi.org/10.1007/978-1-62703-444-9_29

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-443-2

  • Online ISBN: 978-1-62703-444-9

  • eBook Packages: Springer Protocols

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