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Pericyte Coculture Models to Study Astrocyte, Pericyte, and Endothelial Cell Interactions

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Astrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 814))

Abstract

The microvascular pericyte is an integral component of the blood-brain barrier and the neurovascular unit. Most model systems that have been developed to study the functional parameters of these systems have not incorporated the pericyte. In this chapter, we consider pericyte coculture and triple culture systems and detail the methodology, suggestions, and problems with isolation of these unique cells. We also present data to show that triple cultures are ideal to study the role of the CNS pericyte in CNS angiogenesis.

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Acknowledgments

Part of the work discussed in this chapter was supported in part by: Grants from the National Institute of Health NINDS (NS47672) and the National Multiple Sclerosis Society (CA1042A8, PP1517).

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Authors and Affiliations

  1. Department of Neurology, Division of Neuroimmunology, Wayne State University School of Medicine, Detroit, MI, USA

    Vladimir Katyshev & Paula Dore-Duffy

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  1. Vladimir Katyshev
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  2. Paula Dore-Duffy
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Corresponding author

Correspondence to Paula Dore-Duffy .

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Editors and Affiliations

  1. Dept. Molecular & Experimental, Scripps Research Institute, N. Torrey Pines Road 10550, La Jolla, 92037, California, USA

    Richard Milner

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Katyshev, V., Dore-Duffy, P. (2012). Pericyte Coculture Models to Study Astrocyte, Pericyte, and Endothelial Cell Interactions. In: Milner, R. (eds) Astrocytes. Methods in Molecular Biology, vol 814. Humana Press. https://doi.org/10.1007/978-1-61779-452-0_31

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  • DOI: https://doi.org/10.1007/978-1-61779-452-0_31

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-451-3

  • Online ISBN: 978-1-61779-452-0

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