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In Vitro Models of the Blood–Brain Barrier

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Astrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 814))

Abstract

The chapter provides an introduction and brief overview of currently available in vitro blood–brain barrier models, pointing out the major advantages and disadvantages of the respective models and potential applications. Bovine brain microvessel endothelial cell isolation, culture, and transendothelial permeability measurement procedures are discussed in detail as a model system for a laboratory to begin brain vascular investigations.

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Acknowledgments

This work was supported by R01 NS046526 and NS076012.

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Authors and Affiliations

  1. School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA

    Kaushik K. Shah, Li Yang & Thomas J. Abbruscato

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  1. Kaushik K. Shah
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  2. Li Yang
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  3. Thomas J. Abbruscato
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Correspondence to Thomas J. Abbruscato .

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  1. Dept. Molecular & Experimental, Scripps Research Institute, N. Torrey Pines Road 10550, La Jolla, 92037, California, USA

    Richard Milner

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Shah, K.K., Yang, L., Abbruscato, T.J. (2012). In Vitro Models of the Blood–Brain Barrier. In: Milner, R. (eds) Astrocytes. Methods in Molecular Biology, vol 814. Humana Press. https://doi.org/10.1007/978-1-61779-452-0_29

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  • DOI: https://doi.org/10.1007/978-1-61779-452-0_29

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