Abstract
Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies
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References
Böyum, A. (1968) Isolation of mononuclear cells and granulocytes from human blood. Scan. J. Clin. Lab. Invest. 21, 77–89.
Skoog, W. A. and Beck, W. S. (1956) Studies on the fibrinogen, dextran, and phytohemagglutinin methods of isolating leukocytes. Blood 11, 436–454.
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© 2007 Humana Press Inc., Totowa, NJ
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Nauseef, W.M. (2007). Isolation of Human Neutrophils From Venous Blood. In: Quinn, M.T., DeLeo, F.R., Bokoch, G.M. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology™, vol 412. Humana Press. https://doi.org/10.1007/978-1-59745-467-4_2
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DOI: https://doi.org/10.1007/978-1-59745-467-4_2
Publisher Name: Humana Press
Print ISBN: 978-1-58829-788-4
Online ISBN: 978-1-59745-467-4
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