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Cardiomyocytes From Human and Mouse Embryonic Stem Cells

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Tissue Engineering

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 140))

Summary

Human and mouse embryonic stem (ES) cells have the potential to differentiate to cardiomyo- cytes in culture. They are therefore of interest for studying early human and mouse heart development, as well as properties of cardiomyocytes from both species, including their responses to cardiac drugs, and, at some point in the future, may represent a source of transplantable cells for cardiac muscle repair. The differentiation protocols that are effective depend in part on the species from which the ES cell lines were derived, and in part on the individual cell lines and the methods used for their propagation prior to differentiation. Here, several methods for generating and characterizing cardiomyocytes from mouse and human ES cells are described, as well as methods for dissociation of cardiomyocytes into single-cell suspensions which are useful both for characterizing cells by antibody staining and electrophysiological measurements, as well as preparing cells for transplantation into (animal) hearts.

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Author information

Authors and Affiliations

  1. Hubrecht Laboratory, Utrecht, the Netherlands

    Christine Mummery, Robert Passier, Dorien Ward, Stieneke van den Brink, Marga van Rooijen & Anja van de Stolpe

  2. Department of Medical Physiology, University Medical Center Utrecht, Utrecht, the Netherlands

    Marcel A. G. van der Heyden & Teun P. de Boer

Authors
  1. Christine Mummery
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  2. Marcel A. G. van der Heyden
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  3. Teun P. de Boer
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  4. Robert Passier
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  5. Dorien Ward
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  6. Stieneke van den Brink
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  7. Marga van Rooijen
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  8. Anja van de Stolpe
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Editor information

Editors and Affiliations

  1. Department of Gene Regulation and Differentiation, National Research Center for Biotechnology GBF, Braunschweig, Germany

    Hansjörg Hauser

  2. Institute for Chemical and Bio-Engineering, ETH Zurich, Zurich, Switzerland

    Martin Fussenegger

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© 2007 Humana Press Inc.

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Mummery, C. et al. (2007). Cardiomyocytes From Human and Mouse Embryonic Stem Cells. In: Hauser, H., Fussenegger, M. (eds) Tissue Engineering. Methods in Molecular Medicine™, vol 140. Humana Press. https://doi.org/10.1007/978-1-59745-443-8_14

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  • DOI: https://doi.org/10.1007/978-1-59745-443-8_14

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-756-3

  • Online ISBN: 978-1-59745-443-8

  • eBook Packages: Springer Protocols

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