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Measurement of Glycosaminoglycan Release from Cartilage Explants

  • Protocol
Arthritis Research

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 135))

Abstract

Quantitation of glycosaminoglycans (GAGs) in the form of aggrecan fragments released from cartilage in culture is a simple way to determine the efficacy of different cytokines alone or in combination in simulating cartilage catabolism. Two approaches for GAG assay are described, with special attention being paid to the advantages and limitations of each method

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References

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Author information

Authors and Affiliations

  1. Joint Diseases Laboratory, Shriners Hospital for Children, Montreal, Quebec, Canada

    John S. Mort

  2. Genetics Unit, Shriners Hospital for Children and Department of Surgery and Human Genetics, McGill University, Montreal, Quebec, Canada

    Peter J. Roughley

Authors
  1. John S. Mort
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  2. Peter J. Roughley
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Editor information

Editors and Affiliations

  1. The Kennedy Institute of Rheumatology, Imperial College London, London, UK

    Andrew P. Cope

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© 2007 Humana Press Inc., Totowa, NJ

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Mort, J.S., Roughley, P.J. (2007). Measurement of Glycosaminoglycan Release from Cartilage Explants. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 135. Humana Press. https://doi.org/10.1007/978-1-59745-401-8_12

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  • DOI: https://doi.org/10.1007/978-1-59745-401-8_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-344-2

  • Online ISBN: 978-1-59745-401-8

  • eBook Packages: Springer Protocols

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