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Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells

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Cancer Metabolism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1928))

Abstract

Blood vessels in tumors contain chaotic branching structures and leaky vessel lumens, resulting in uneven supply of oxygen in the tumor microenvironment. High metabolic and proliferation rate of tumor cells further depletes the local oxygen supply. Therefore, hypoxia is a common phenomenon in multiple solid malignancies. Hypoxia-inducible factors (HIFs) regulate the transcription of a spectrum of genes, which are vitally important for tumor cell adaption under hypoxia, and shape the tumor microenvironment to become more favorable for progression. HIFs are involved in almost every step of cancer development through inducing angiogenesis, metabolic reprogramming, metastasis, cancer stemness maintenance, chemoresistance, and immune evasion. Here, we describe methods for the assessment of HIF activity, as well as identification of novel transcriptional targets of HIFs in vitro and in vivo.

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  1. David Kung-Chun Chiu, Misty Shuo Zhang, and Aki Pui-Wah Tse contributed equally to this work.

Authors and Affiliations

  1. Department of Pathology, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong

    David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse & Carmen Chak-Lui Wong

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  1. David Kung-Chun Chiu
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  2. Misty Shuo Zhang
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  3. Aki Pui-Wah Tse
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Correspondence to Carmen Chak-Lui Wong .

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  1. National Cancer Institute, North Bethesda, MD, USA

    Majda Haznadar

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Chiu, D.KC., Zhang, M.S., Tse, A.PW., Wong, C.CL. (2019). Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells. In: Haznadar, M. (eds) Cancer Metabolism. Methods in Molecular Biology, vol 1928. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9027-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9027-6_6

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