Abstract
Chikungunya is a mosquito-borne infection with clinical presentation of fever, arthralgia, and rash. The etiological agent Chikungunya virus (CHIKV) is generally transmitted from primates to humans through the bites of infected Aedes aegypti and Aedes albopictus mosquitoes. Outbreaks of Chikungunya occur commonly with varied morbidity, mortality, and sequele according to the epidemiological, ecological, seasonal, and geographical impact. Investigations are required to be conducted as a part of the public health service to understand and report the suspected cases as confirmed by laboratory diagnosis. Holistic sampling at a time of different types would be useful for laboratory testing, result conclusion, and reporting in a valid way. The use of serum samples for virus detection, virus isolation, and serology is routinely practiced, but sometimes serum samples from pediatric and other cases may not be easily available. In such a situation, easily available throat swabs and urine samples could be useful. It is already well reported for measles, rubella, and mumps diseases to have the virus diagnosis from throat swabs and urine. Here, we present the protocols for diagnosis of CHIKV using throat swab and urine specimens.
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Acknowledgement
This work supported by the National Institute of Virology, Pune, the National Vector Borne Disease Control Programme, New Delhi, and the State Health Department, Government of Karnataka. Thanks are due to Mr. D. P. Sinha and M. J. Manjunath for systematic technical works.
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Raut, C.G., Hanumaiah, H., Raut, W.C. (2016). Utilization and Assessment of Throat Swab and Urine Specimens for Diagnosis of Chikungunya Virus Infection. In: Chu, J., Ang, S. (eds) Chikungunya Virus. Methods in Molecular Biology, vol 1426. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3618-2_7
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DOI: https://doi.org/10.1007/978-1-4939-3618-2_7
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