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Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface

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Rhinoviruses

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1221))

Abstract

Rhinovirus-C (RV-C) were discovered recently using molecular methods. Classical methods failed to detect them since they could not grow in standard cell culture. The complete genome sequences of several RV-C strains are now available but there is little information about their biological characteristics. HRV-C were first grown in organ culture, and more recently, we developed a system for culturing RV-C strains in differentiated epithelial cells of human airway at air-liquid interface (ALI). These cultures supported efficient replication of RV-C strains as determined by quantitative RT-PCR. This system has enabled study of the biological characteristics of RV-C strains, including quantitative research.

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Acknowledgements

Funding for development of the ALI tissue culture system for RV-C was provided by NIH grant U19 AI070503.

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Authors and Affiliations

  1. Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA

    Shamaila Ashraf

  2. American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, VA, 20109, USA

    Shamaila Ashraf

  3. Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA

    Rebecca Brockman-Schneider

  4. Department of Pediatrics and Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA

    James E. Gern

Authors
  1. Shamaila Ashraf
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  2. Rebecca Brockman-Schneider
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  3. James E. Gern
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Corresponding author

Correspondence to Shamaila Ashraf .

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Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Health, Nursing and Medical Sciences,, Monash University, Melbourne, Victoria, Australia

    David A. Jans

  2. Centre for Research in Therapeutic Solutions, Faculty of Education, Science, Technology and Mathematics, University of Canberra, Canberra, Aust Capital Terr, Australia

    Reena Ghildyal

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Ashraf, S., Brockman-Schneider, R., Gern, J.E. (2015). Propagation of Rhinovirus-C Strains in Human Airway Epithelial Cells Differentiated at Air-Liquid Interface. In: Jans, D., Ghildyal, R. (eds) Rhinoviruses. Methods in Molecular Biology, vol 1221. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1571-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-1571-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1570-5

  • Online ISBN: 978-1-4939-1571-2

  • eBook Packages: Springer Protocols

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