Abstract
Up until 1970, it was not understood why the deproteinized genomic RNAs of positive-strand viruses such as poliovirus were infectious while those of negative-strand viruses such as vesicular stomatitis virus (VSV) were not. An explanation of this difference was obtained when Baltimore et al. (1970) incubated highly purified virions of VSV with nonionic detergent, salts, and nucleoside triphosphates and demonstrated that RNA was synthesized in vitro. There was an RNA-dependent RNA polymerase packaged in the virion! Since the RNA synthesized in vitro was complementary to the genomic RNA, it was thought to be messenger RNA (mRNA), and the enzyme responsible for its synthesis was named the “transcriptase.” It is now clear that the obligatory first biosynthetic step in the infectious cycles of negative-strand viruses is transcription. Since the host cells appear to lack enzymes capable of utilizing RNA as templates, the infecting virus particle must carry its own supply of transcriptase into the cell to initiate the viral reproductive cycle. It is generally assumed that the viral polymerase is totally or mostly coded for by viral genes.
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References
Abraham, G., and Banerjee, A. K., 1976, Sequential transcription of the genes of vesicular stomatitis virus, Proc. Natl. Acad. Sci. U.S.A. 73: 1504–1508.
Ball, L. A., and White, C. N., 1976, Order of transcription of genes on vesicular stomatitis virus, Proc. Natl. Acad. Sci. U.S.A. 73: 442–446.
Baltimore, D., Huang, A. S., and Stampfer, M., 1970, Ribonucleic acid synthesis of vesicular stomatitis virus. II. An RNA polymerase in the virion, Proc. Natl. Acad. Sci. U.S.A. 66: 572–576.
Banerjee, A. K., Abraham, G, and Colonno, R. J., 1977, Vesicular stomatitis virus: Mode of transcription, J. Gen. Virol. 34: 1–8.
Banerjee, A. K., De, B. P., and Sanchez, A., 1985, Transcription of vesicular stomatitis virus genome RNA, Viral Messenger RNA (Y. Becker, ed.), pp. 197–224, Martinus Nijhoff Publishing, Boston.
Bell, J. C., Brown E. G., Takayesu, D., and Prevec, L., 1984, Protein kinase activity associated with immunoprecipitates of the vesicular stomatitis virus phosphoprotein NS, Virology 132: 229–238.
Belle-Isle, H. D., and Emerson, S. U., 1982, Use of a hybrid infectivity assay to analyze primary transcription of temperature-sensitive mutants of the New Jersey serotype of vesicular stomatitis virus, J. Virol. 43: 37–40.
Bishop, D. H. L., and Roy, P., 1972, Dissociation of vesicular stomatitis virus and relation of the virion proteins to the viral transcriptase, J. Virol. 10: 234–243.
Blumberg, B. M., Giorgi, C., and Kolakofsky, D., 1983, N protein of vesicular stomatitis virus selectively encapsidates leader RNA in vitro, Cell 32: 559–567.
Blumberg, B. M., Giorgi, C., Rose, K., and Kolakofsky, D., 1984, Preparation and analysis of the nucleocapsid proteins of vesicular stomatitis virus and Sendai virus, and analysis of the Sendai virus leader—NP gene region, J. Gen. Virol. 65: 769–779.
Carroll, A. R., and Wagner, R. R. 1978, Inhibition of transcription by immunoglobulins directed against the ribonucleoprotein of homotypic and heterotypic vesicular stoma-titis virus. J. Virol. 25: 675–684.
Chanda, P. K., and Banerjee, A. K., 1981, Identification of promoter-proximal oligonucleotides, and a unique oligonucleotide pppGpC from in vitro transcription products of vesicular stomatitis virus, J. Virol. 39: 93–103.
Chinchar, V. G., Amesse, L. S., and Portner, A., 1982, Linked transcripts of the genes for leader and N message are synthesized in vitro by vesicular stomatitis virus, Biochem. Biophys. Res. Commun. 105: 1296–1302.
Clinton, G. M., Burge, B. W., and Huang, A. S., 1978, Effects of phosphorylation and pH on the association of NS protein with vesicular stomatitis virus cores, J. Virol. 27: 340–346.
Clinton, G. M., Guerina, N. G., Guo, H., and Huang, A. S., 1982, Host dependent phosphorylation and kinase activity associated with vesicular stomatitis virus. J. Biol. Chem. 257: 3313–3319.
Colonno, R. J., and Banerjee, A. K., 1976, A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro, Cell 8: 197–204.
Colonno, R. J., and Banerjee, A. K., 1977, Mapping and initiation studies on the leader RNA of vesicular stomatitis virus, Virology 77: 260–268.
De, B. P., and Banerjee, A. K., 1984, Specific interactions of vesicular stomatitis virus L and NS proteins with heterologous genome ribonucleoprotein template lead to mRNA synthesis in vitro, J. Virol. 51: 628–634.
De, B. P., and Banerjee, A. K., 1985, Requirements and functions of vesicular stomatitis virus L and NS proteins in the transcription process in vitro, Biochem. Biophys. Res. Commun. 126: 40–49.
De, B. P., Tahara, S. M., and Banerjee, A. K., 1982, Production and characterization of monoclonal antibody to the N protein of vesicular stomatitis virus (Indiana serotype), Virology 122: 510–514.
Deutsch V., Muel, B., and Brun, G., 1979, Temperature-sensitive mutant is 082 of vesicular stomatitis virus. 1. Rescue at non-permissive temperature by UV-irradiated virus, Virology 93: 286–290.
Emerson, S. U., 1982, Reconstitution studies detect a single polymerase entry site on the vesicular stomatitis virus genome, Cell 31: 635–642.
Emerson, S. U., and Wagner, R. R., 1972, Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions, J. Virol. 10: 297–309.
Emerson, S. U., and Wagner, R. R., 1973, L protein requirement for in vitro RNA synthesis by vesicular stomatitis virus, J. Virol. 12: 1325–1335.
Emerson, S. U., and Yu, Y. H., 1975, Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus, J. Virol. 15: 1348–1356.
Emerson, S. U., Dierks, P. M., and Parsons, J. T., 1977, In vitro synthesis of a unique RNA species by a T particle of vesicular stomatitis virus, J. Virol. 23: 708–716.
Flamand, A., 1980, Rhabdovirus genetics, in: Rhabdoviruses, Vol. II ( D. H. L. Bishop, ed.), pp. 115–140, CRC Press, Boca Raton, Florida.
Gallione, C. J., Greene, J. R., Iverson, L. E., and Rose, J. K., 1981, Nucleotide sequences of the mRNAs encoding the vesicular stomatitis virus N and NS proteins, J. Virol. 39: 529–535.
Giorgi, C., Blumberg, B., and Kolakofsky, D., 1983, Sequence determination of the (+ j leader RNA regions of the vesicular stomatitis virus Chandipura, Cocal, and Piry serotype genomes, J. Virol. 46: 125–130.
Green, T. L., and Emerson, S. U., 1984, Effect of the beta—gamma phosphate bond of ATP on synthesis of leader RNA and mRNAs of vesicular stomatitis virus, J. Virol. 50: 255–257.
Harmon, S. A., and Summers, D. F., 1982, Characterization of monospecific antisera against all five vesicular stomatitis virus-specific proteins: Anti-L and anti-NS inhibit transcription in vitro, Virology 120: 194–204.
Harmon, S. A., Marnell, L. L., and Summers, D. F., 1983, The major ribonucleoprotein associated protein kinase of vesicular stomatitis virus is a host cell protein, J. Biol Chem. 258:15, 283–15, 290.
Harmon, S. A., Robinson, E. N., Jr., and Summers, D. F., 1985, Ultrastructural localization of L and NS enzyme subunits on vesicular stomatitis virus RNPs using gold sphere—staphylococcal protein A—monospecific IgG conjugates, Virology 142: 406–410.
Herman, R. C., Adler, S., Lazzarini, R. A., Colonno, R. J. Banerjee, A. K., and Westphal, H., 1978, Intervening polyadenylate sequences in RNA transcripts of vesicular stomatitis virus, Cell 15: 587–596.
Horikami, S. M., and Moyer, S. A., 1982, Host range mutants of vesicular stomatitis virus defective in in vitro RNA methylation, Proc. Natl. Acad. Sci. U.S.A. 79: 7694–7698.
Horikami, S. M., DeFerra, F., and Moyer, S. A., 1984, Characterization of the infections of permissive and nonpermissive cells by host range mutants of vesicular stomatitis virus defective in RNA methylation, Virology 138: 1–15.
Hsu, C. H., and Kingsbury, D. W., 1982, NS phosphoprotein of vesicular stomatitis virus: Subspecies separated by electrophoresis and isoelectric focusing, J. Virol. 42: 342–345.
Hsu, C. H., Morgan, E. M., and Kingsbury, D. W., 1982, Site-specific phosphorylation regulates the transcriptive activity of vesicular stomatitis virus NS protein, J. Virol. 43: 104–112.
Hunt, D. M., 1983, Vesicular stomatitis virus mutant with altered polyadenylic acid polymerase activity in vitro, J. Virol. 46: 788–799.
Hunt, D. M., Emerson, S. U., and Wagner, R. R., 1976, RNA-temperature-sensitive mutants of vesicular stomatitis virus: L protein thermosensitivity accounts for transcriptase restriction of group I mutants, J. Virol. 18: 596–603.
Hunt, D. M., Smith, E. F., and Buckley, D. W., 1984, Aberrant polyadenylation by a vesicular stomatitis virus mutant is due to an altered L protein, J. Virol. 52: 515–521.
Imblum, R. L., and Wagner, R. R., 1974a, Inhibition of viral transcriptase by immunoglobulin directed against the nucleocapsid NS protein of vesicular stomatitis virus, J. Virol. 15: 1357–1366.
Imblum, R. L., and Wagner, R. R., 1974b, Protein kinase and phosphoproteins of vesicular stomatitis virus, J. Virol. 13: 113–124.
Isaac, C. L., and Keene, J. D., 1982, RNA polymerase-associated interactions near template promoter sequences of defective interfering particles of vesicular stomatitis virus, J. Virol. 43: 241–249.
Iverson, L. E., and Rose, J. K., 1982, Sequential synthesis of 5’-proximal vesicular stomatitis virus mRNA sequences, J. Virol. 44: 356–365.
Keene, J. D., Schubert, M., and Lazzarini, R. A., 1979, Terminal sequences of vesicular stomatitis virus RNA are both complementary and conserved, J. Virol. 32: 167–174.
Keene, J. D., Thornton, B. J., and Emerson, S. U. 1981, Sequence-specific contacts between the RNA polymerase of vesicular stomatitis virus and the leader RNA gene, Proc. Natl. Acad. Sci. U.S.A. 78: 6191–6195.
Kingsbury, D. W., Hsu, C. H., and Morgan, E. M., 1981, A role for NS-protein phosphorylation in vesicular stomatitis virus transcription, in: The Replication of Negative Strand Viruses ( D. H. L. Bishop and R. W. Compans, eds.), pp. 821–827, Elsevier/North-Holland, Amsterdam.
Kingsford, L., and Emerson, S. U., 1980, Transcriptional activities of different phosphorylated species of NS protein purified from vesicular stomatitis virus and cytoplasm of infected cells, J. Virol. 33: 1097–1105.
Lazzarini, R. A., Keene, J. D., and Schubert, M., 1981, The origins of defective interfering particles of the negative-strand RNA viruses, Cell 26: 145–154.
Marnell, L. L., and Summers, D. F., 1984, Characterization of the phosphorylated small enzyme subunit, NS, of the vesicular stomatitis virus RNA polymerase, J. Biol. Chem. 259:13,518–13,524.
Mellon, M. G., and Emerson, S. U., 1978, Rebinding of transcriptase components (L and NS proteins) the nucleocapsid template of vesicular stomatitis virus, J. Virol. 27: 560–567.
Naeve, C. E., and Summers, D. F., 1981, Initiation of transcription by vesicular stomatitis virus occurs at multiple sites, in: The Replication of Negative Strand Viruses ( D. H. L. Bishop and R. W. Compans, eds.), pp. 769–779, Elsevier/North-Holland, Amsterdam.
Naito, S., and Ishihama, A., 1976, Function and structure of RNA polymerase from vesicular stomatitis virus, J. Biol. Chem. 251: 4307–4314.
Nakai, T., and Howatson, A. F., 1968, The fine structure of vesicular stomatitis virus, Virology 35: 268–281.
Pinney, D. F., and Emerson, S. U., 1982a, Identification and characterization of a group of discrete initiated oligonucleotides transcribed in vitro from the 3’ terminus of the N-gene of vesicular stomatitis virus, J. Virol. 42: 889–896.
Pinney, D. F., and Emerson, S. U., 1982b, In vitro synthesis of triphosphate-initiated N-gene mRNA oligonucleotides is regulated by the matrix protein of vesicular stomatitis virus, J. Virol. 42: 897–904.
Piwnica-Worms, H., and Keene, J. D., 1983, Sequential synthesis of small capped RNA transcripts in vitro by vesicular stomatitis virus, Virology 125: 206–218.
Pringle, C. R., 1982, The genetics of vesiculoviruses, Arch. Virol. 72: 1–34.
Rose, J. K., Lodish, H. F., and Brock, M. L., 1977, Giant heterogeneous polyadenylic acid on vesicular stomatitis virus mRNA synthesized in vitro in the presence of S-adenosylhomocysteine, J. Virol. 21: 683–693.
Sanchez, A., De, B. P., and Banerjee, A. K., 1985, In vitro phosphorylation of NS by the L protein of vesicular stomatitis virus, J. Gen. Virol. 66: 1025–1036.
Schubert, M., and Lazzarini, R. A., 1981, In vivo transcription of the 5’-terminal extracistronic regions of vesicular stomatitis virus RNA, J. Virol. 38: 256–262.
Schubert, M., Keene, J. D., Lazzarini, R. A., and Emerson, S. U., 1978, The complete sequence of a unique RNA species synthesized by a DI particle of VSV, Cell 15: 103–112.
Schubert, M., Keene, J. D., Herman, R. C., and Lazzarini, R. A., 1980, Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA, J. Virol. 34: 550–559.
Schubert, M., Harmison, G. G., Sprague, J., Condra, C. S., and Lazzarini, R. A., 1982, In vitro transcription of vesicular stomatitis virus: Initiation with GTP at a specific site within the N cistron, J. Virol. 43: 166–173.
Schubert, M., Harmison, G. G., and Meier, E., 1984, Primary structure of vesicular stomatitis virus polymerase (L) gene: Evidence for a high frequency of mutations, J. Virol. 51: 505–514.
Schubert, M., Harmison, G. G., and Richardson, C. D., and Meier, E., 1985, Expression of a cDNA encoding a functional, 241 kilodalton vesicular stomatitis virus RNA polymerase, Proc. Natl. Acad. Sci. U.S.A. 82: 7984–7988.
Sinacore, M. S., and Lucas-Lenard, J., 1982, The effect of the vesicular stomatitis virusassociated protein kinase on viral mRNA transcription in vitro, Virology 121: 404–413.
Szilagyi, J. F., and Uryvayev, L., 1973, Isolation of an infectious ribonucleoprotein from VSV containing an active RNA transcriptase, J. Virol. 11: 279–286.
Talib, S., and Hearst, J. E., 1983, Initiation of RNA synthesis in vitro by vesicular stomatitis virus: Single internal initiation in the presence of aurintricarboxylic acid and vanadyl ribonucleoside complexes, Nucleic Acids Res. 11: 7031–7042.
Testa, D., and Banerjee, A. K., 1977, Two methyltransferase activities in the purified virions of vesicular stomatitis virus, J. Virol. 24: 786–793.
Testa, D., and Banerjee, A. K., 1979, Initiation of RNA synthesis in vitro by vesicular stomatitis virus, J. Biol. Chem. 254: 2053–2058.
Testa, D., Chanda, P. K., and Banerjee, A. K., 1980a, Unique mode of transcription in vitro by vesicular stomatitis virus, Cell 21: 267–275.
Testa, D., Chanda, P. K., and Banerjee, A. K., 1980b, In vitro synthesis of the full-length complement of the negative-strand genome RNA of vesicular stomatitis virus, Proc. Natl. Acad. Sci. U.S.A. 77: 294–298.
Thomas, D., Newcomb, W. W., Brown, J. C., Wall, J. S., Hainfeld, J. F., Trus, B. L., and Steven, A. C., 1985, Mass and molecular composition of vesicular stomatitis virus: A STEM analysis, J. Virol. 54: 598–607.
Thornton, G. B., De, B. P., and Banerjee, A. K., 1984, Interaction of L and NS proteins of vesicular stomatitis virus with its template ribonucleoprotein during RNA synthesis in vitro, J. Gen. Virol. 65: 663–668.
Villarreal, L. P., Briendl, M., and Holland, J. J., 1976, Determination of molar ratios of vesicular stomatitis virus induced RNA species in BHK-21 cells, Biochemistry 15: 1663–1667.
Wagner, R., 1975, Reproduction of rhabdoviruses, in: Comprehensive Virology, Vol. 4 ( H. Fraenkel-Conrat and R. Wagner, eds.), pp. 1–93, Plenum Press, New York.
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© 1987 Springer Science+Business Media New York
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Emerson, S.U. (1987). Transcription of Vesicular Stomatitis Virus. In: Wagner, R.R. (eds) The Rhabdoviruses. The Viruses. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-7032-1_6
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DOI: https://doi.org/10.1007/978-1-4684-7032-1_6
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