Abstract
Evidence has accumulated for a number of years that rapid membrane-initiated steroid signaling can occur (1;2) in conjunction with the binding of steroids to cellular surfaces (3). However, the identification of the class of protein which mediates this binding, and initiates the accompanying signals, has remained controversial. Our earlier collaborative studies on the membrane glucocorticoid receptors (see chapter 6) demonstrated that a monoclonal antibody (Ab) to the intracellular form of that protein could be used as a tool to identify and study an additional form of the receptor on the cell surface; the presence of this receptor could be correlated with a subset of glucocorticoid-initiated responses. Subsequently, we looked for an appropriate, rapidly estrogen-responsive cell model in which to investigate the presence and identity of a membrane estrogen receptor linked to nongenomic actions.
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Watson, C., Zivadinovic, D., Bulayeva, N., Hawkins, B., Campbell, C., Gametchu, B. (2003). A Membrane Form of Estrogen Receptor-α Mediates Estrogenic, Nongenomic Effects. In: Watson, C.S. (eds) The Identities of Membrane Steroid Receptors. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0339-2_2
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DOI: https://doi.org/10.1007/978-1-4615-0339-2_2
Publisher Name: Springer, Boston, MA
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