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Basic Principles of Posttranslational Modification of Proteins and Their Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

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Two-Dimensional Electrophoresis and Immunological Techniques

Abstract

Many of the biological activities and physicochemical properties of proteins can be attributed to their posttranslational modifications, including glycosylation (the addition of sugar residues), sulfation (the addition of SO4 groups), or phosphorylation (the addition of PO4 residues). One of the most important uses of high-resolution two-dimensional polyacrylamide gel electrophoresis (PAGE) is for analysis of such modifications, which frequently cause shifts in the charge and thus the migration of proteins. It is therefore important to have a basic understanding of these modifications if the analysis methods described in this text are to be used.

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  1. Baylor College of Medicine, Houston, Texas, USA

    Bonnie S. Dunbar

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Dunbar, B.S. (1987). Basic Principles of Posttranslational Modification of Proteins and Their Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis. In: Two-Dimensional Electrophoresis and Immunological Techniques. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1957-3_5

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  • DOI: https://doi.org/10.1007/978-1-4613-1957-3_5

  • Publisher Name: Springer, Boston, MA

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