Abstract
Two-dimensional paper chromatography represents one of the best methods for the rapid separation of mixtures of flavonoids from crude methanol or methanol-water extracts of dried plant material. Furthermore, sufficient quantities of the separated compound for both hydrolytic and ultraviolet spectral analyses often can be isolated from about 30 chromatograms. The present discussion will emphasize the chromatographic techniques routinely employed in this laboratory for the analysis of flavonoids; however, a number of other procedures and variations have been published elsewhere [1, 2]
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Seikel, M. K., in: The Chemistry of Flavonoid Compounds (edited by T. A. Geissman ), p. 34–69. Oxford: Pergamon Press 1962.
a) Harborne, J. B.: J. Chromatography 1, 473 (1958). b) Harbome, J. B.: J. Chromatography 2, 581 (1959).
Markham, K. R., and T. J. Mabry: Phytochemistry 7, 791 (1968).
a) Thomas, M. B., and T. J. M.bry: Phytochemistry 7, 787 (1968). b) Seeligman, P., and R. E. Alston: Brittonia 19, 205 (1967).
Wallace, J.W., Jr.: Ph. D. Dissertation, University of Texas at Austin, 1967, “Investigations of Flavone Biosynthesis in the Lemnaceae ”.
Alston, R. E., in: Recent Advances in Phytochemistry (edited by T. J. Mabry, R. E. Alston, and V. C. Runeckles), p. 305 — 327. New York: Appleton-Century-Crofts 1968.
Markham, K. R., T. J. Mabry, and W.T. Swift III: Phytochemistry 7, 803 (1968).
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1970 Springer-Verlag New York Inc.
About this chapter
Cite this chapter
Mabry, T.J., Markham, K.R., Thomas, M.B. (1970). The Two-Dimensional Paper Chromatographic Analysis of Flavonoids. In: The Systematic Identification of Flavonoids. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-88458-0_1
Download citation
DOI: https://doi.org/10.1007/978-3-642-88458-0_1
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-88460-3
Online ISBN: 978-3-642-88458-0
eBook Packages: Springer Book Archive