Abstract
A pleural effusion is the abnormal accumulation of fluid between the two layers of pleura that line the chest cavity and surround the lung. Pleural effusion can be the result of several causes. Proteomic analysis may be useful in indicating the pathogenic mechanisms involved in pleural fluid accumulation and might pinpoint specific diagnosis. Differential gel electrophoresis (DIGE) has been employed to compare directly the proteome profile of serum and pleural effusion of mesothelioma patients to identify unique proteins by observing concentration changes and modifications on the single protein level. This technique involves the preelectrophoretic labeling of complex protein samples using different cyanine-based fluorescent tags prior to carrying out separation by 2D polyacrylamide gel electrophoresis. Several proteins have been found to be differentially or uniquely expressed in the serum or pleural effusion of patients.
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© 2007 Humana Press Inc., Totowa, NJ
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Hegmans, J., Hemmes, A., Lambrecht, B. (2007). Proteomics of Pleural Effusion. In: Thongboonkerd, V. (eds) Proteomics of Human Body Fluids. Humana Press. https://doi.org/10.1007/978-1-59745-432-2_13
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DOI: https://doi.org/10.1007/978-1-59745-432-2_13
Publisher Name: Humana Press
Print ISBN: 978-1-58829-657-3
Online ISBN: 978-1-59745-432-2
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