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01-11-2024 | Vulgar Psoriasis | Original Paper

Recombinant ISRAA ameliorates imiquimod-induced psoriasis and knocking out Israa delays its onset

Authors: Manahel Mahmood Alsabbagh, Muna Aljishi, Ameera Sultan, Amar Muhsin Marwani, Nasneen Althaf, Moiz Bakhiet, Safa Taha

Published in: Archives of Dermatological Research | Issue 9/2024

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Abstract

Psoriasis, an inflammatory disease, is largely mediated by T-helper 17 cytokines. We have previously identified the immune system-released activating agent (Israa) as a novel gene that connects the nervous and immune systems. This research aims to investigate the role of the Israa gene in psoriasis in vivo using the imiquimod-induced psoriasis model. We established the model in C57BL/6 wildtype mice, which were then treated with 200 pg/mouse, 400 pg/mouse, or 800 pg/mouse of recombinant ISRAA compared to methotrexate. Subsequently, we also induced psoriasis in Israa-knockout mice to confirm the effect of Israa. Results consistently showed improvement in psoriasis in all groups receiving recombinant ISRAA. The 200 pg/mouse dose eliminated the disease, reduced the cutaneous release of IL-17 to one-third and TNF-α to one-sixth, increased IL-10 release to over 500 pg, completely resolved parakeratosis, decreased epidermal thickness to one-half, and reduced the expression of CD4 and neutrophil elastase in the skin (all p < 0.05). Israa-knockout mice exhibited less severe psoriasis in all scoring, biochemical, and histological parameters compared to wild-type mice (p < 0.05). This study highlights Israa as a crucial molecule in psoriasis and confirms its immunomodulatory role in inflammatory diseases.
Literature
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Metadata
Title
Recombinant ISRAA ameliorates imiquimod-induced psoriasis and knocking out Israa delays its onset
Authors
Manahel Mahmood Alsabbagh
Muna Aljishi
Ameera Sultan
Amar Muhsin Marwani
Nasneen Althaf
Moiz Bakhiet
Safa Taha
Publication date
01-11-2024
Publisher
Springer Berlin Heidelberg
Published in
Archives of Dermatological Research / Issue 9/2024
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI
https://doi.org/10.1007/s00403-024-03410-5

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