Skip to main content
Top
Published in:

16-01-2024 | Ulcerative Colitis | Original Article

Baricitinib relieves DSS-induced ulcerative colitis in mice by suppressing the NF-κB and JAK2/STAT3 signalling pathways

Authors: Qiong Wu, Yangyang Liu, Jinmeiqi Liang, Ao Dai, Boyu Du, Xueyan Xi, Lan Jin, Yang Guo

Published in: Inflammopharmacology | Issue 1/2024

Login to get access

Abstract

Ulcerative colitis (UC) is a relapsing inflammatory disease with a unique aetiology. The treatment of UC is challenging, and the current clinical therapeutics for colitis have limited efficacy. Thus, finding new and effective treatment options remains urgent. Baricitinib, an inhibitor of Janus kinase (JAK), has been clinically used to treat rheumatoid arthritis (RA). However, its potential effects on UC have not been fully elucidated. In this study, we aimed to explore the effects of baricitinib on UC and its underlying mechanism. Dextran sulphate sodium (DSS)-induced murine model of chronic colitis was used to investigate the intervention efficacy following oral administration of baricitinib. The levels of key cytokines, such as IL-6, IFN-γ and IL-17A, were determined. Moreover, western blotting for IκBα, p-IκBα, JAK2, p-JAK2, STAT3 and p-STAT3 protein expression was performed to investigate the associated signalling pathways. Our findings demonstrated that baricitinib can significantly relieve DSS-induced UC in mice. After baricitinib intervention, IL-6, IFN-γ and IL-17A levels were decreased both in vitro and in vivo. Moreover, the elevated expression levels of p-IκBα, p-JAK2, and p-STAT3 were significantly reduced after treatment. Collectively, these results suggest that baricitinib is a potential therapeutic agent for alleviation of DSS-induced colitis. This study provides a method for subsequent investigations on potential curative drugs development of the for colitis.
Literature
go back to reference Murata Y, Ishiguro Y, Itoh J et al (1995) The role of proinflammatory and immunoregulatory cytokines in the pathogenesis of ulcerative colitis. J Gastroenterol 30(Suppl 8):56–60PubMed Murata Y, Ishiguro Y, Itoh J et al (1995) The role of proinflammatory and immunoregulatory cytokines in the pathogenesis of ulcerative colitis. J Gastroenterol 30(Suppl 8):56–60PubMed
Metadata
Title
Baricitinib relieves DSS-induced ulcerative colitis in mice by suppressing the NF-κB and JAK2/STAT3 signalling pathways
Authors
Qiong Wu
Yangyang Liu
Jinmeiqi Liang
Ao Dai
Boyu Du
Xueyan Xi
Lan Jin
Yang Guo
Publication date
16-01-2024
Publisher
Springer International Publishing
Published in
Inflammopharmacology / Issue 1/2024
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI
https://doi.org/10.1007/s10787-023-01396-6

Other articles of this Issue 1/2024

Inflammopharmacology 1/2024 Go to the issue