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Open Access 03-01-2024 | Tuberculosis | Brief Report

Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients

Authors: Isaac Acheampong, Difery Minadzi, Edwin F. Laing, Michael Frimpong, Monika M. Vivekanandan, Augustine Yeboah, Ernest Adankwah, Wilfred Aniagyei, Joseph F. Arthur, Millicent Lamptey, Mohammed K. Abass, Francis Kumbel, Francis Osei-Yeboah, Amidu Gawusu, Linda Batsa Debrah, Dorcas O. Owusu, Alexander Debrah, Ertan Mayatepek, Julia Seyfarth, Richard O. Phillips, Marc Jacobsen

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 3/2024

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Abstract

Impaired T-cell responses to mitogens and high T-cell activation marker (TAM) expression on Mycobacterium tuberculosis–specific T-cells characterize immunopathology in patients with tuberculosis (TB). In a study of patients with TB (n = 60) and asymptomatic contacts (controls, n = 37), we found that TB patients had higher CD38+ T-cell proportions specific for M. tuberculosis protein (PPDMtb), yet total proportions of PPDMtb-specific T-cells were comparable. Notably, both activated (CD38+) and total IFN-γ+ T-cells from TB patients had lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired mitogen response improved the classification efficacy of the TAM-TB assay, especially employing the PPD/PHA-induced T-cell ratio.
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Metadata
Title
Differences in PPD- and mitogen-induced T-cell activation marker expression characterize immunopathology in acute tuberculosis patients
Authors
Isaac Acheampong
Difery Minadzi
Edwin F. Laing
Michael Frimpong
Monika M. Vivekanandan
Augustine Yeboah
Ernest Adankwah
Wilfred Aniagyei
Joseph F. Arthur
Millicent Lamptey
Mohammed K. Abass
Francis Kumbel
Francis Osei-Yeboah
Amidu Gawusu
Linda Batsa Debrah
Dorcas O. Owusu
Alexander Debrah
Ertan Mayatepek
Julia Seyfarth
Richard O. Phillips
Marc Jacobsen
Publication date
03-01-2024
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 3/2024
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-023-04741-3

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