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Journal of Endocrinological Investigation
Published in:

01-04-2023 | Thyroid Cancer | Original Article

Silencing circ_0000644 inhibits papillary thyroid cancer cell malignancy by combining with miR-671-5p to release the inhibition on ANXA2

Authors: Hong-Guang Li, Li-Hong Zhao, Jun-Zhao Liu, Kun-Peng Liu, Jian-Bo Liu, Zi-Jie Su, Yong-Ju Gao

Published in: Journal of Endocrinological Investigation | Issue 4/2023

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Abstract

Background

Papillary thyroid cancer (PTC) is life-threatening due to its malignant progression. Considerable evidence demonstrates that circular RNA (circRNA) regulates PTC development. This study aims to explore the mechanism of circ_0000644 modulating PTC malignant progression.

Methods

The RNA levels of circ_0000644, microRNA-671-5p (miR-671-5p) and annexin A2 (ANXA2) were detected by quantitative real-time polymerase chain reaction. Western blot was performed to check protein expression. Cell proliferation and cell apoptosis were investigated by 5-ethynyl-29-deoxyuridine and flow cytometry. Angiogenic capacity, migration and invasion were analyzed by tube formation assay and transwell assay. The interaction between miR-671-5p and circ_0000644 or ANXA2 was identified by dual-luciferase reporter assay. Xenograft mouse model assay was performed to analyze the effect of circ_0000644 on tumor formation in vivo.

Results

Circ_0000644 and ANXA2 expression was significantly upregulated, while miR-671-5p was downregulated in PTC tissues and cells when compared with control groups. Circ_0000644 knockdown inhibited PTC cell proliferation, tube formation, migration, and invasion, but induced apoptosis in vitro. Moreover, circ_0000644 knockdown led to delayed tumorigenesis in vivo. In addition, circ_0000644 acted as a miR-671-5p sponge and mediated PTC cell tumor properties through miR-671-5p. ANXA2 was identified as a target gene of miR-671-5p, and its overexpression relieved miR-671-5p-induced effects in PTC cells. Furthermore, circ_0000644 depletion inhibited ANXA2 production by combining with miR-671-5p.

Conclusion

Circ_0000644 depletion repressed PTC cell tumor properties through the miR-671-5p/ANXA2 axis.
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Literature
1.
Kitahara CM, Sosa JA (2016) The changing incidence of thyroid cancer. Nat Rev Endocrinol 12:646–653CrossRefPubMed
2.
3.
Clarke CA, Reynolds P, Oakley-Girvan I, Lee E, Lu Y, Yang J et al (2015) Indicators of microbial-rich environments and the development of papillary thyroid cancer in the California teachers study. Cancer Epidemiol 39:548–553CrossRefPubMedPubMedCentral
4.
Guo M, Sun Y, Ding J, Li Y, Yang S, Zhao Y et al (2021) Circular RNA profiling reveals a potential role of hsa_circ_IPCEF1 in papillary thyroid carcinoma. Mol Med Rep 24:603CrossRefPubMedPubMedCentral
5.
6.
7.
Thomson DW, Dinger ME (2016) Endogenous microRNA sponges: evidence and controversy. Nat Rev Genet 17:272–283CrossRefPubMed
8.
Kristensen LS, Andersen MS, Stagsted LVW, Ebbesen KK, Hansen TB, Kjems J (2019) The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet 20:675–691CrossRefPubMed
9.
10.
Lou W, Ding B, Wang J, Xu Y (2020) The involvement of the hsa_circ_0088494-miR-876-3p-CTNNB1/CCND1 axis in carcinogenesis and progression of papillary thyroid carcinoma. Front Cell Dev Biol 8:605940CrossRefPubMedPubMedCentral
11.
Wen X, Du J, Wang X (2021) Circ_0039411 promotes papillary thyroid carcinoma development through mediating the miR-423-5p/SOX4 signaling. Int J Biol Markers 36:10–20CrossRefPubMed
12.
Yao Y, Chen X, Yang H, Chen W, Qian Y, Yan Z et al (2019) Hsa_circ_0058124 promotes papillary thyroid cancer tumorigenesis and invasiveness through the NOTCH3/GATAD2A axis. J Exp Clin Cancer Res 38:318CrossRefPubMedPubMedCentral
13.
Nie C, Han J, Bi W, Qiu Z, Chen L, Yu J et al (2022) Circular RNA circ_0000644 promotes papillary thyroid cancer progression via sponging miR-1205 and regulating E2F3 expression. Cell Cycle 21:126–139CrossRefPubMed
14.
15.
Zembska A, Jawiarczyk-Przybyłowska A, Wojtczak B, Bolanowski M (2019) MicroRNA expression in the progression and aggressiveness of papillary thyroid carcinoma. Anticancer Res 39:33–40CrossRefPubMed
16.
Jiang YM, Liu W, Jiang L, Chang H (2021) CircLDLR promotes papillary thyroid carcinoma tumorigenicity by regulating miR-637/LMO4 axis. Dis Mark 2021:3977189
17.
Yu S, Cao S, Hong S, Lin X, Guan H, Chen S et al (2019) miR-3619-3p promotes papillary thyroid carcinoma progression via Wnt/β-catenin pathway. Ann Transl Med 7:643CrossRefPubMedPubMedCentral
18.
Wang WJ, Yuan Y, Zhang D, Liu P, Liu F (2021) miR-671-5p repressed progression of papillary thyroid carcinoma via TRIM14. Kaohsiung J Med Sci 37:983–990CrossRefPubMed
19.
20.
He Q, Zhao L, Liu X, Zheng J, Liu Y, Liu L et al (2019) MOV10 binding circ-DICER1 regulates the angiogenesis of glioma via miR-103a-3p/miR-382-5p mediated ZIC4 expression change. J Exp Clin Cancer Res 38:9–9CrossRefPubMedPubMedCentral
21.
Huang D-W, Huang M, Lin X-S, Huang Q (2017) CD155 expression and its correlation with clinicopathologic characteristics, angiogenesis, and prognosis in human cholangiocarcinoma. Onco Targets Ther 10:3817–3825CrossRefPubMedPubMedCentral
22.
23.
Wang H, Yan X, Zhang H, Zhan X (2019) CircRNA circ_0067934 overexpression correlates with poor prognosis and promotes thyroid carcinoma progression. Med Sci Monit 25:1342–1349CrossRefPubMedPubMedCentral
24.
25.
26.
Nelson AR, Fingleton B, Rothenberg ML, Matrisian LM (2000) Matrix metalloproteinases: biologic activity and clinical implications. J Clin Oncol 18:1135–1149CrossRefPubMed
27.
Tan X, Fu Y, Chen L, Lee W, Lai Y, Rezaei K et al (2016) miR-671-5p inhibits epithelial-to-mesenchymal transition by downregulating FOXM1 expression in breast cancer. Oncotarget 7:293–307CrossRefPubMed
28.
Song H, Xu Y, Xu T, Fan R, Jiang T, Cao M et al (2020) CircPIP5K1A activates KRT80 and PI3K/AKT pathway to promote gastric cancer development through sponging miR-671-5p. Biomed Pharmacother 126:109941CrossRefPubMed
29.
Zhu Z, Luo L, Xiang Q, Wang J, Liu Y, Deng Y et al (2020) MiRNA-671-5p promotes prostate cancer development and metastasis by targeting NFIA/CRYAB axis. Cell Death Dis 11:949CrossRefPubMedPubMedCentral
30.
31.
Ma S, Lu CC, Yang LY, Wang JJ, Wang BS, Cai HQ et al (2018) ANXA2 promotes esophageal cancer progression by activating MYC-HIF1A-VEGF axis. J Exp Clin Cancer Res 37:183CrossRefPubMedPubMedCentral
32.
Xie R, Liu J, Yu X, Li C, Wang Y, Yang W et al (2019) ANXA2 Silencing inhibits proliferation, invasion, and migration in gastric cancer cells. J Oncol 2019:4035460CrossRefPubMedPubMedCentral
33.
Hong W, Ying H, Lin F, Ding R, Wang W, Zhang M (2020) lncRNA LINC00460 silencing represses EMT in colon cancer through downregulation of ANXA2 via upregulating miR-433-3p. Mol Ther Nucleic Acids 19:1209–1218CrossRefPubMed
34.
Li HG, Zhao LH, Lu A, Liu JB, Su ZJ, Wang XB et al (2021) [The mechanism of circ_0023990/miR-873–5p/ANXA2 axis regulating radiosensitivity and development of thyroid carcinoma]. Zhonghua Yi Xue Za Zhi 101:3329–3337PubMed
35.
Qin YY, Huang SN, Chen G, Pang YY, Li XJ, Xing WW et al (2020) Clinicopathological value and underlying molecular mechanism of annexin A2 in 992 cases of thyroid carcinoma. Comput Biol Chem 86:107258CrossRefPubMed
Metadata
Title
Silencing circ_0000644 inhibits papillary thyroid cancer cell malignancy by combining with miR-671-5p to release the inhibition on ANXA2
Authors
Hong-Guang Li
Li-Hong Zhao
Jun-Zhao Liu
Kun-Peng Liu
Jian-Bo Liu
Zi-Jie Su
Yong-Ju Gao
Publication date
01-04-2023
Publisher
Springer International Publishing
Published in
Journal of Endocrinological Investigation / Issue 4/2023
Electronic ISSN: 1720-8386
DOI
https://doi.org/10.1007/s40618-022-01930-3

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