Skip to main content
Key Specialties
Anesthesiology Cardiology Dermatology Diabetology Endocrinology Gastroenterology General Medicine Geriatrics Hematology Infectious Diseases Internal Medicine Nephrology Neurology Obstetrics and Gynecology Oncology Pediatrics Psychiatry Radiology Respiratory Medicine Rheumatology Surgery Urology
Congress Coverage
2024 ESMO Congress 2024 ERS Congress 2024 EASD Congress 2024 ESC Congress 2024 EAN Congress 2024 ASCO Annual Meeting 2024 ACC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on modern management of frailty

LIVE: Thursday 17th October 2024, 18:00-19:30 (CEST)

Frailty has a significant impact on health and wellbeing, especially in older adults. Join our experts to understand the factors that contribute to the development of frailty and find out how to manage the condition and reduce the risk of disability, dependency, and mortality.
ADVANCED SEARCH
Log in
Top
European Spine Journal

06-09-2024 | Original Article

The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus fibrosus

Authors: Tianchi Zhou, Bowei Xiao, Juying Huang, Tianhua Rong, Bingxuan Wu, Baoge Liu

Published in: European Spine Journal

Login to get access

Abstract

Purpose

To compare the mechanical properties of human annulus fibrosus obtained by forceps versus bistoury and observe whether the measurement could be affected by forceps sampling method.

Methods

In this study, the mechanical properties of the the extracellular matrix (ECM) of human annulus fibrosus, including elastic modulus and stiffness, were investigated using atomic force microscope (AFM). Tissue was obtained from patients during operation using a bistoury or nucleus pulposus forceps. Tissues obtained with the nucleus pulposus forceps were considered as the forceps group and those obtained with a bistoury were considered as the bistoury group.

Results

There was no significant difference observed between the forceps and bistoury group according to histological staining. The elastic modulus of the forceps group was 0.41 ± 0.08 MPa, and that of bistoury group was 0.53 ± 0.13 MPa, and the difference between the two groups was statistically significant (p < 0.05). The stiffness of the forceps group was 0.024 ± 0.003 N/m, and that of the bistoury group was 0.037 ± 0.003 N/m, and the difference between the two groups was statistically significant (p < 0.05).

Conclusion

The results indicate that the forceps sampling method has a substantial negative effect on the micromechanical properties of the ECM of the annulus fibrosus. Bistoury sampling method is recommended as the experimental subject for exploring the micromechanics mechanisms of cervical degenerative disease.
Literature
1.
Reilly GC, Knapp HF, Stemmer A, Neiderer P, Knothe-Tate ML (2001) Investigation of the morphology of the lacunocanalicular system of cortical bone using atomic force microscopy. Ann Biomed Eng 29(12):1074–1081CrossRefPubMed
2.
Xu T, Fu R, Yan L (2003) A new insight into the adsorption of bovine serumalbumin onto porous polyethylene membrane by Zeta potential measurements, FTIR analysis, and AFM observations. J Colloid Interface Sci 262(2):342–350CrossRefPubMed
3.
Hengsburger S, Kulik A, Zyssett P (2002) Nanoindentation discriminates the elastic properties of individual human bone lamellae under dry and physiological conditions. Bone 30(1):178–184CrossRef
4.
Kinney JH, Habelitz S, Marshall SJ, Marshall GW (2003) The importance of intrafibrillar mineralization of collagen on the mechanical properties of dentin. J Dent Res 82(12):957–961CrossRefPubMed
5.
Jurvelin JS, Muller DJ, Wong M, Studer D, Engel A, Hunziker EB (1996) Surface and subsurface morphology of bovine humeral articular cartilage as assessed by atomic force and transmission electron microscopy.J. Struct Biol 117:45–54CrossRef
6.
Danalache M, Jacobi LF, Schwitalle M, Hofmann UK. Assessment of biomechanical properties of the extracellular and pericellular matrix and their interconnection throughout the course of osteoarthritis. J Biomech. :97:109409.
7.
Patel R, JJ (2019) 2003. Microstructural and elastic properties of the extracellular matrices of the superficial zone of neonatal articular cartilage by atomic force microscopy. Front. Biosci. 1 (8), 18–25
8.
Gabrielii I, Gatenholm P (1998) Preparation and properties of hydrogels based on hemicellulose. J Appl Polym Sci 69:1661–1667CrossRef
9.
Ambrosio L, De Santis R, Nicolais L (1998) Composite hydrogels for implants. Proc. Inst. Mech. Eng. 212 (H), 93–99
10.
Ashton-Miller JA, Schultz AB (1997) Biomechanics of the human spine. In: Mow VC, Hayes WC (eds) Basic Orthopaedic Biomechanics, Second edn. Lippincott-Raven, Philadelphia, PA, pp 353–385
11.
Humzah MD, Soames RW (1988) Human intervertebral disc: structure and function. Anat Rec 220(4):337–356CrossRefPubMed
12.
Lewis NT, Hussain MA, Mao JJ (2008) Investigation of nano-mechanical properties of annulus fibrosus using atomic force microscopy. Micron 39(7):1008–1019CrossRefPubMed
13.
Tavakoli J, Elliott DM, Costi JJ (2017) The ultra-structural organization of the elastic network in the intra- and inter-lamellar matrix of the intervertebral disc. Acta Biomater 58:269–277CrossRefPubMed
14.
Codan B, Martinelli V, Mestroni L, Sbaizero O (2013) Atomic force microscopy of 3T3 and SW-13 cell lines: an investigation of cell elasticity changes due to fixation. Mater Sci Eng C Mater Biol Appl 33(6):3303–3308CrossRefPubMed
15.
Jastrzebska M, Barwinski B, Mróz I, Turek A, Zalewska-Rejdak J, Cwalina B (2005) Atomic force microscopy investigation of chemically stabilized pericardium tissue. Eur Phys J E Soft Matter 16(4):381–388CrossRefPubMed
16.
Graham HK, Hodson NW, Hoyland JA, Millward-Sadler SJ, Garrod D, Scothern A, Griffiths CE, Watson RE, Cox TR, Erler JT, Trafford AW, Sherratt MJ (2010) Tissue section AFM: in situ ultrastructural imaging of native biomolecules. Matrix Biol 29(4):254–260CrossRefPubMedPubMedCentral
17.
Humzah MD, Soames RW (1988) Human intervertebral disc: structure and function. Anat Rec Hob 220(4):337–356CrossRef
18.
Chu G, Shi C, Lin J, Wang S, Wang H, Liu T et al (2018) Biomechanics in annulus fibrosus degeneration and regeneration. Adv Exp Med Biol 1078:409–420CrossRefPubMed
19.
Bibby SR, Jones DA, Lee RB, Yu J, Urban JP (2001) The pathophysiology of the intervertebral disc. Jt Bone Spine 68(6):537–542CrossRef
20.
Roberts S, Menage J, Duance V, Wotton S, Ayad S (1991) 1991 Volvo Award in basic sciences. Collagen types around the cells of the intervertebral disc and cartilage end plate: an immunolocalization study. Spine (Phila Pa 1976) 16(9):1030–1038CrossRefPubMed
21.
22.
Song Y, Zhao B, Zhang L, Lü J, Wang S, Dong Y, Hu J (2014) The origin of the snap-in in the force curve between AFM probe and the water/gas interface of nanobubbles. ChemPhysChem 15(3):492–499CrossRefPubMed
23.
Rico F, Wojcikiewicz EP, Moy VT (2008) Atomic force microscopy studies of the mechanical properties of living cells, in: Appl. Scanning Probe Methods IX, NanoScience and Technology, Ed. Springer-Verlag, N. Y
24.
Granzier HL, Pollack GH 2000. Elastic filaments of the cell. Springer Science Busisness Media LLC
25.
26.
Thompson JP, Pearce RH, Schechter MT, Adams ME, Tsang IK, Bishop PB (1990) Preliminary evaluation of a scheme for grading the gross morphology of the human intervertebral disc. Spine (Phila Pa 1976) 15(5):411–415CrossRefPubMed
27.
Best BA, Guilak F, Setton LA, Zhu W, Saed-Nejad F, Ratcliffe A, Weidenbaum M, Mow VC (1994) Compressive mechanical properties of the human anulus fibrosus and their relationship to biochemical composition. Spine 19(2):212–221CrossRefPubMed
28.
Berthet-Colominas C, Miller A, Herbage D, Ronziere M-C, Tocchetti D (1982) Structural studies of collagen fibres from intervertebral disc. Biochim Biophys Acta - Protein Struct Mol Enzym 706(1):50–64CrossRef
29.
Metadata
Title
The mechanical properties measurement could be affected by forceps: a technical note on sampling human annulus fibrosus
Authors
Tianchi Zhou
Bowei Xiao
Juying Huang
Tianhua Rong
Bingxuan Wu
Baoge Liu
Publication date
06-09-2024
Publisher
Springer Berlin Heidelberg
Published in
European Spine Journal
Print ISSN: 0940-6719
Electronic ISSN: 1432-0932
DOI
https://doi.org/10.1007/s00586-024-08438-9