Skip to main content
Key Specialties
Anesthesiology Cardiology Dermatology Diabetology Endocrinology Gastroenterology General Medicine Geriatrics Hematology Infectious Diseases Internal Medicine Nephrology Neurology Obstetrics and Gynecology Oncology Pediatrics Psychiatry Radiology Respiratory Medicine Rheumatology Surgery Urology
Congress Coverage
2024 ERS Congress 2024 EASD Congress 2024 ESC Congress 2024 EAN Congress 2024 ASCO Annual Meeting 2024 ACC Congress
Clinical Collections
Advances in Alzheimer’s

ERS Congress 2024

Keep up to date on the latest news from the annual European Respiratory Society conference.
ADVANCED SEARCH
Log in
Top
Journal of Assisted Reproduction and Genetics
Published in:

16-05-2024 | Gamete Biology

The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation

Authors: Rahil Jannatifar, Hamid Piroozmanesh, Seyedeh Saeideh Sahraei, Atefeh Verdi, Elham Asa

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2024

Login to get access

Abstract

Aim

The aim of this study is the evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.

Methods

Semen samples of 50 Asthenoteratozoospermia men (random) were collected. Sperm parameters were analyzed based on World Health Organization (WHO, 2010) criteria (2021) and each sample was divided into 5 groups (E1–E5). E1 (control group): the sperm was cryopreserved without nanoliposome, and Mito-Tempo. E2: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.1 mM) + freezing medium. E3: sperm cryopreservation with Mito-Tempo-loaded nanoliposome (Mito-Tempo 0.2 mM) + freezing medium. E4: in this group, the cryopreservation sperm with Mito-Tempo 0.3 mM + freezing medium. E5: the cryopreservation sperm with Mito-Tempo 0.2 mM + freezing medium.

Results

The result of this study indicated that sperm parameters and total antioxidant capacity (TAC) significantly increase in E3 and E4 groups, compared to E1, E2, and E5 groups respectively (P < 0.05). The percentage of abnormal morphology, DNA fragmentation index (DFI), malondialdehyde (MDA), and the levels of ROS significantly decrease in E3 and E4 groups, compared to E1, E2, and E5 groups (P < 0.05). In addition, the sperm parameters and stress oxidative factors significantly improve in E3 group compared to other groups (P < 0.05).

Conclusions

In conclusion, the combination of Mito-Tempo with nanoliposome due to its ability to cooperate with lipid layers may lead to significant performance in reducing oxidative stress damage and increasing the quality of sperm parameters.
Literature
1.
2.
3.
4.
Cheng Q, Li L, Jiang M, Liu B, Xian Y, Liu S, et al. Extend the survival of human sperm in vitro in non-freezing conditions: damage mechanisms, preservation technologies, and clinical applications. Cells. 2022;11(18):2845.PubMedPubMedCentralCrossRef
5.
Agarwal A, Farkouh Aa, Saleh R, Hamoda TA-AA-M, Harraz AM, Kavoussi P, et al. Controversy and consensus on indications for sperm DNA fragmentation testing in male infertility: a global survey, current guidelines, and expert recommendations. World J Men’s Health. 2023;41(3):575.CrossRef
6.
Motlagh MK, Sharafi M, Zhandi M, Mohammadi-Sangcheshmeh A, Shakeri M, Soleimani M, et al. Antioxidant effect of rosemary (Rosmarinus officinalis L.) extract in soybean lecithin-based semen extender following freeze–thawing process of ram sperm. Cryobiology. 2014;69(2):217–22.PubMedCrossRef
7.
Lu X, Zhang Y, Bai H, Liu J, Li J, Wu B. Mitochondria-targeted antioxidant MitoTEMPO improves the post-thaw sperm quality. Cryobiology. 2018;80:26–9.PubMedCrossRef
8.
Jannatifar R, Asa E, Sahraei SS, Verdi A, Piroozmanesh H. N-acetyl‐l‐cysteine and alpha lipoic acid are protective supplement on human sperm parameters in cryopreservation of asthenoteratozoospermia patients. Andrologia. 2022;54(11):e14612.PubMedCrossRef
9.
Zhang X, Lu X, Li J, Xia Q, Gao J, Wu B. Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients. Cryobiology. 2019;91:18–22.PubMedCrossRef
10.
Wang P-F, Xie K, Cao Y-X, Zhang A. Hepatoprotective effect of mitochondria-targeted antioxidant mito-TEMPO against lipopolysaccharide-induced liver injury in mouse. Mediators of inflammation. 2022;2022.
11.
SAYLAN A, DIRAMALI M. The effects of Mito-TEMPO, a mitochondria targeted antioxidant, on frozen human sperm parameters. Turkish J Health Sport Volume. 2023;4(1):1.
12.
Jannatifar R, Parivar K, Roodbari NH, Nasr-Esfahani MH. Effects of N-acetyl-cysteine supplementation on sperm quality, chromatin integrity and level of oxidative stress in infertile men. Reproductive Biology Endocrinol. 2019;17(1):1–9.CrossRef
13.
Najafi A, Kia HD, Mehdipour M, Hamishehkar H, Álvarez-Rodríguez M. Effect of quercetin loaded liposomes or nanostructured lipid carrier (NLC) on post-thawed sperm quality and fertility of rooster sperm. Theriogenology. 2020;152:122–8.PubMedCrossRef
14.
Bhaskar S, Tian F, Stoeger T, Kreyling W, de la Fuente JM, Grazú V, et al. Multifunctional nanocarriers for diagnostics, drug delivery and targeted treatment across blood-brain barrier: perspectives on tracking and neuroimaging. Part Fibre Toxicol. 2010;7(1):1–25.CrossRef
15.
Patra JK, Das G, Fraceto LF, Campos EVR, Rodriguez-Torres MdP, Acosta-Torres LS, et al. Nano based drug delivery systems: recent developments and future prospects. J Nanobiotechnol. 2018;16(1):1–33.CrossRef
16.
Liu R, Luo C, Pang Z, Zhang J, Ruan S, Wu M, et al. Advances of nanoparticles as drug delivery systems for disease diagnosis and treatment. Chin Chem Lett. 2023;34(2):107518.CrossRef
17.
Iqbal MA, Md S, Sahni JK, Baboota S, Dang S, Ali J. Nanostructured lipid carriers system: recent advances in drug delivery. J Drug Target. 2012;20(10):813–30.PubMedCrossRef
18.
Garg N, Ahmad FJ. Epigallocatechin-3-gallate-loaded nanocarriers for health benefits. Nanomedicine for Bioactives: Healthcare applications. 2020:393–411.
19.
Huang S-L. Liposomes in ultrasonic drug and gene delivery. Adv Drug Deliv Rev. 2008;60(10):1167–76.PubMedCrossRef
20.
Eloy JO, de Souza MC, Petrilli R, Barcellos JPA, Lee RJ, Marchetti JM. Liposomes as carriers of hydrophilic small molecule drugs: strategies to enhance encapsulation and delivery. Colloids Surf B. 2014;123:345–63.CrossRef
21.
Martínez-Ballesta M, Gil-Izquierdo Á, García-Viguera C, Domínguez-Perles R. Nanoparticles and controlled delivery for bioactive compounds: outlining challenges for new smart-foods for health. Foods. 2018;7(5):72.PubMedPubMedCentralCrossRef
22.
Organization WH. WHO laboratory manual for the examination and processing of human semen. World Health Organization; 2021.
23.
Ziarati N, Topraggaleh TR, Rahimizadeh P, Montazeri L, Maroufizadeh S, Gilani MAS, et al. Micro-quantity straw as a carrier for cryopreservation of oligozoospermic semen samples: effects of storage times and cryoprotectant. Cryobiology. 2019;86:65–70.PubMedCrossRef
24.
Quintela A, Oliveira I, Souza A, Gusmão A, Silva A. Water-induced hypo-osmotic test for the evaluation of canine sperm membrane integrity. Anim Reprod (AR). 2018;7(2):70–4.
25.
Kheirollahi-Kouhestani M, Razavi S, Tavalaee M, Deemeh M, Mardani M, Moshtaghian J, et al. Selection of sperm based on combined density gradient and Zeta method may improve ICSI outcome. Hum Reprod. 2009;24(10):2409–16.PubMedCrossRef
26.
Keller A, Mohamed A, Dröse S, Brandt U, Fleming I, Brandes RP. Analysis of dichlorodihydrofluorescein and dihydrocalcein as probes for the detection of intracellular reactive oxygen species. Free Radic Res. 2004;38(12):1257–67.PubMedCrossRef
27.
Marchetti C, Jouy N, Leroy-Martin B, Defossez A, Formstecher P, Marchetti P. Comparison of four fluorochromes for the detection of the inner mitochondrial membrane potential in human spermatozoa and their correlation with sperm motility. Hum Reprod. 2004;19(10):2267–76.PubMedCrossRef
28.
Zhu Z, Li R, Lv Y, Zeng W. Melatonin protects rabbit spermatozoa from cryo-damage via decreasing oxidative stress. Cryobiology. 2019;88:1–8.PubMedCrossRef
29.
Hassan MA, Khalil WA, Abdelnour SA, Aman RM. Supplementation of alpha-lipoic acid-loaded nanoliposomes in semen extender improves freezability of buffalo spermatozoa. Sci Rep. 2022;12(1):22464.PubMedPubMedCentralCrossRef
30.
Naidoo S. Studies on factors influencing viability after cryopreservation of excised zygotic embryos from recalcitrant seeds of two amaryllid species 2010.
31.
Lembo D, Donalisio M, Civra A, Argenziano M, Cavalli R. Nanomedicine formulations for the delivery of antiviral drugs: a promising solution for the treatment of viral infections. Expert Opin Drug Deliv. 2018;15(1):93–114.PubMedCrossRef
32.
Pateiro M, Gómez B, Munekata PE, Barba FJ, Putnik P, Kovačević DB, et al. Nanoencapsulation of promising bioactive compounds to improve their absorption, stability, functionality and the appearance of the final food products. Molecules. 2021;26(6):1547.PubMedPubMedCentralCrossRef
33.
Alfei S. Nanotechnology applications to improve solubility of bioactive constituents of foods for health-promoting purposes. Nano-food Engineering: Volume One. 2020:189–257.
34.
Wijekoon MJO, Mahmood K, Ariffin F, Nafchi AM, Zulkurnain M. Recent advances in encapsulation of fat-soluble vitamins using polysaccharides, proteins, and lipids: a review on delivery systems, formulation, and industrial applications. Int J Biol Macromol. 2023:124539.
35.
Tabibiazar M, Mohammadifar MA, Roufegarinejad L, Ghorbani M, Hashemi M, Hamishehkar H. Improvement in dispersibility, stability and antioxidant activity of resveratrol using a colloidal nanodispersion of BSA-resveratrol. Food Bioscience. 2019;27:46–53.CrossRef
36.
Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, et al. Mitochondria-targeted triphenylphosphonium-based compounds: syntheses, mechanisms of action, and therapeutic and diagnostic applications. Chem Rev. 2017;117(15):10043–120.PubMedPubMedCentralCrossRef
37.
Atayik MC, Çakatay U. Mitochondria-targeted senotherapeutic interventions. Biogerontology. 2022;23(4):401–23.PubMedCrossRef
38.
Partyka A, Niżański W. Supplementation of avian semen extenders with antioxidants to improve semen quality—is it an effective strategy? Antioxidants. 2021;10(12):1927.PubMedPubMedCentralCrossRef
39.
Shi L, Shi J, Feng J, Zhang P, Ren Y. Proteomic analysis reveals the potential positive effects of Mito-TEMPO on ram sperm motility and fertility during cryopreservation. Theriogenology. 2023;205:27–39.PubMedCrossRef
40.
Zidni I, Lee HB, Yoon JH, Park JY, Oh YD, Jang HS, et al. Effect of antioxidants in cryopreservation media on spotted halibut (Verasfer Variegatus) sperm quality during cryopreservation. Aquaculture. 2022;557:738351.CrossRef
41.
Vaughn AK. Mitochondrial formate production and its impact on embryonic cranial tissue development in the mouse. The University of Texas at Austin; 2019.
42.
Lemma A. Effect of cryopreservation on sperm quality and fertility. Artif Insemin farm Anim. 2011;12:191–216.
43.
Dadras H, Golpour A, Rahi D, Lieskovská J, Dzyuba V, Gazo I, et al. Cryopreservation of sterlet, Acipenser ruthenus spermatozoa: evaluation of quality parameters and fine ultrastructure. Front Mar Sci. 2022;9:783278.CrossRef
44.
Kumar A, Kumar Ghosh S, Katiyar R, Gemeda AE, Rautela R, Bisla A, et al. Supplementation of Mito TEMPO and acetovanillone in semen extender improves freezability of buffalo spermatozoa. Andrology. 2022;10(4):775–88.PubMedCrossRef
45.
Asadzadeh N, Abdollahi Z, Esmaeilkhanian S, Masoudi R. Fertility and flow cytometry evaluations of ram frozen semen in plant-based extender supplemented with Mito-TEMPO. Anim Reprod Sci. 2021;233:106836.PubMedCrossRef
46.
Zarei F, Kia HD, Masoudi R, Moghaddam G, Ebrahimi M. Supplementation of ram’s semen extender with Mito-TEMPO I: improvement in quality parameters and reproductive performance of cooled-stored semen. Cryobiology. 2021;98:215–8.PubMedCrossRef
47.
Aitken RJ. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev. 2017;84(10):1039–52.PubMedCrossRef
48.
Liu Y, Perumal E, Bi X, Wang Y, Ding W. Potential mechanisms of uremic muscle wasting and the protective role of the mitochondria-targeted antioxidant Mito-TEMPO. Int Urol Nephrol. 2020;52:1551–61.PubMedCrossRef
49.
50.
Ríos J-L, Giner RM, Marín M, Recio MC. A pharmacological update of ellagic acid. Planta Med. 2018;84(15):1068–93.PubMedCrossRef
51.
Du Plessis SS, Agarwal A, Halabi J, Tvrda E. Contemporary evidence on the physiological role of reactive oxygen species in human sperm function. J Assist Reprod Genet. 2015;32:509–20.PubMedPubMedCentralCrossRef
52.
Jannatifar R, Piroozmanesh H, Jannatifar Z. Effect of N-acetylcysteine on human sperm parameters and DNA damage in frozen-thawed sperm samples of asthenozoospermic men. Qom Univ Med Sci J. 2021;14(12):32–40.CrossRef
Metadata
Title
The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation
Authors
Rahil Jannatifar
Hamid Piroozmanesh
Seyedeh Saeideh Sahraei
Atefeh Verdi
Elham Asa
Publication date
16-05-2024
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 8/2024
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-024-03132-7

Other articles of this Issue 8/2024

Journal of Assisted Reproduction and Genetics 8/2024 Go to the issue

Assisted Reproduction Technologies

Outcomes of oocyte retrievals completed by reproductive endocrinology and infertility fellows versus faculty: a 7-year retrospective review

Reproductive physiology and disease

Idiopathic secondary azoospermia occurrence in men with oligospermia over time

Assisted Reproduction Technologies

Best quality vs. sex selection – an analysis of embryo selection preferences for patients undergoing preimplantation genetic testing for aneuploidy over a 10-year period

Assisted Reproduction Technologies

Optimized sperm selection: a highly efficient device for the isolation of progressive motile sperm with low DNA fragmentation index

Genetics

Novel PATL2 variants cause female infertility with oocyte maturation defect

Commentary

Genetic factors of oocyte maturation arrest: an important cause for recurrent IVF/ICSI failures