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European Journal of Medical Research
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Open Access 01-12-2017 | Research

The effect of lipoaspirates vs. dissected abdominal fat on breast cancer cells in vitro

Authors: Faris Almarzouqi, Hans-Oliver Rennekampff, Jan-Philipp Stromps, Ziyad Alharbi, Norbert Pallua

Published in: European Journal of Medical Research | Issue 1/2017

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Abstract

Background

Cancer cells are typically surrounded by stromal cells and embedded in extracellular matrix (ECM). The stromal compartment interacts with cancer cells to promote growth and metastasis. For decades, autologous fasciocutaneous flaps have been safely applied for breast reconstruction after mastectomy. In contrast, the safety of fat grafting (lipofilling) procedure has been under debate regarding the risk of cancer recurrence.

Methods

Harvested fat tissue (lipoaspirates) and dissected abdominal fat (DAF) were co-cultured with MCF-7 breast cancer cells. The vitality of MCF-7 cells was measured using AlamarBlue® consecutively for 5 days. ECM degradation was determined by detection of matrix metalloproteinase-1 (MMP-1) expression in MCF-7 cells. Integrin α2 was measured by Western blot to assess the degree of adhesion and motility of MFC-7 cells.

Results

The MCF-7 proliferation increased substantially when co-cultured with fat tissue. However, there was no significant difference between the proliferation stimulating effects of lipoaspirates and DAF. Similarly, MMP-1 protein expression was equally elevated in MCF-7 cells by both lipoaspirates and DAF. Importantly, MCF-7 cells showed an increased level of integrin α2 once co-cultured with either lipoaspirates or DAF.

Conclusion

Fat tissue increases the proliferation of MCF-7 cells in vitro. Our data suggest that lipoaspirates as well as DAF might possess a considerable potency to promote tumorigenic growth of breast cancer cells. Thus, clinical trials are needed to address the safety of lipofilling by breast reconstruction surgery after mastectomy.
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Metadata
Title
The effect of lipoaspirates vs. dissected abdominal fat on breast cancer cells in vitro
Authors
Faris Almarzouqi
Hans-Oliver Rennekampff
Jan-Philipp Stromps
Ziyad Alharbi
Norbert Pallua
Publication date
01-12-2017
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue 1/2017
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/s40001-017-0251-3

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