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Open Access 01-12-2024 | Research

The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis

Authors: Jingxin Zhou, Jia Zhang, Dan Zhu, Wentong Ma, Qing Zhong, Qin Shen, Jing Su

Published in: BMC Pediatrics | Issue 1/2024

Abstract

Objective

To investigate the diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis (IM).

Methods

A total of 135 children with IM as the IM group and 100 healthy volunteers as the healthy group were included in this retrospective study. Peripheral blood lymphocyte subsets marked as CD3+, CD4+, CD8+, CD16 + CD56+, and CD19 + in the peripheral blood were quantified using flow cytometry. Statistical analysis was performed using the chi-square test, Kruskal-Wallis test, AUROC curve, and Kappa consistency test to assess the diagnostic value of these markers in IM.

Results

The AUROC curve for CD8 + cells and for CD4+/CD8 + ratios both achieved a value of 1 with the sensitivity and specificity of 100% (P<0.001). The Kappa coefficients were 1 for CD8+, CD4+/CD8 + ratios and the combined EBV analysis, indicating a 100% consistency with the clinical diagnosis. Significant differences were also observed in the CD3+, CD4+, CD16 + CD56+, and CD19 + lymphocyte subsets between the IM group and the healthy group (P<0.05).

Conclusion

The evaluation of CD8 + and CD4+/CD8 + ratios in peripheral blood lymphocytes represents a significant advancement in the diagnosis of IM. Peripheral blood lymphocyte testing offers a reliable, sensitive, and specific diagnostic tool to enhance the clinical management of children with IM.

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Title: The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis
Authors: Jingxin Zhou
Jia Zhang
Dan Zhu
Wentong Ma
Qing Zhong
Qin Shen
Jing Su
Publication date: 01-12-2024
Publisher: BioMed Central
Published in: BMC Pediatrics / Issue 1/2024
Electronic ISSN: 1471-2431
DOI: https://doi.org/10.1186/s12887-024-05228-6

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The diagnostic value of peripheral blood lymphocyte testing in children with infectious mononucleosis

Abstract

Objective

Methods

Results

Conclusion

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Abstract

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