Published in:

22-09-2022 | Original Paper

Structure–activity relationship of a housefly neuroprotective dodecapeptide that activates the nuclear factor erythroid 2-related factor 2 pathway

Authors: Sichen Zhang, Chunguo Qian, Hailing Li, Zhimin Zhao, Jianchun Xian, Depo Yang

Published in: Journal of Natural Medicines | Issue 1/2023

Abstract

Neuroprotective antioxidants, especially peptide-based antioxidants, are effective against oxidative stress in neurodegenerative disorders. In this study, we measured the neuroprotective effects of the antioxidant peptide DFTPVCTTELGR (DR12) from housefly Musca domestica L. pupae. Treatment of PC12 and HT22 cells with DR12 significantly reduced glutamate-induced cytotoxicity. Peptide DR12 appeared to exert its neuroprotective effects by attenuating production of reactive oxygen species and malonaldehyde, upregulating the endogenous antioxidants superoxide dismutase and glutathione, and reversing the loss of mitochondrial membrane potential. In addition, DR12 treatment activated the nuclear factor erythroid 2-related factor 2/heme oxygenase-1 signaling pathway. Structure–activity analysis indicated that the superior neuroprotective function of DR12 was related to its cysteine residue. In summary, DR12 may be an attractive therapeutic peptide or precursor to treat neurodegenerative diseases.

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Title: Structure–activity relationship of a housefly neuroprotective dodecapeptide that activates the nuclear factor erythroid 2-related factor 2 pathway
Authors: Sichen Zhang
Chunguo Qian
Hailing Li
Zhimin Zhao
Jianchun Xian
Depo Yang
Publication date: 22-09-2022
Publisher: Springer Nature Singapore
Published in: Journal of Natural Medicines / Issue 1/2023
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-022-01650-1

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Structure–activity relationship of a housefly neuroprotective dodecapeptide that activates the nuclear factor erythroid 2-related factor 2 pathway

Abstract

Other articles of this Issue 1/2023

Humulus lupulus L. extract and its active constituent xanthohumol attenuate oxidative stress and nerve injury induced by iron overload via activating AKT/GSK3β and Nrf2/NQO1 pathways

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Abstract

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Humulus lupulus L. extract and its active constituent xanthohumol attenuate oxidative stress and nerve injury induced by iron overload via activating AKT/GSK3β and Nrf2/NQO1 pathways

Essential oil composition of Curcuma species and drugs from Asia analyzed by headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry

Andrographolide ameliorates hepatic steatosis by suppressing FATP2-mediated fatty acid uptake in mice with nonalcoholic fatty liver disease

Total syntheses of surugamides and thioamycolamides toward understanding their biosynthesis

Correction: Anti-influenza A virus activity of flavonoids in vitro: a structure–activity relationship

Anti-influenza A virus activity of flavonoids in vitro: a structure–activity relationship