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European Archives of Paediatric Dentistry

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09-05-2024 | Streptococci | Original Scientific Article

Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study

Authors: T. S. Shid-Moosavi, N. Mohammadi, Y. Gharamani, M. Motamedifar, A. A. Alizadeh

Published in: European Archives of Paediatric Dentistry | Issue 3/2024

Abstract

Purpose

This study aimed to evaluate the antibacterial and cytotoxic effects of reinforced zinc oxide–eugenol (rZOE) incorporated with different concentrations of silver nanoparticles (AgNPs).

Methods

The pastes of rZOE alone or mixed with AgNPs at concentrations of 1%, 2%, and 5% of weight were prepared. In vitro antimicrobial activity of prepared materials against Streptococcus (S.) mutans and Lactobacillus (L.) acidophilus were evaluated after 2, 4, and 6 h of contact times using direct contact test (DCT) and also following 24 h incubation by well-diffusion test (WDT). The cytotoxicity of the tested materials on human dental pulp stem cells was also determined by MTT assay.

Results

The DCT demonstrated that the time-dependent reductions of the colony numbers of both bacteria by three different concentrations of AgNPs incorporated into rZOE were equal but steeper than the rZOE alone (P < 0.05). The increases in growth inhibition zones of S. mutans and L. acidophilus were associated with the increasing concentration of AgNPs mixed with rZOE in the WDT; however, statistical analysis did not show any significant differences (P = 0.092). The MTT assay revealed a significantly lower percentage of cell viability after 1 day of culture only with the rZOE + AgNP5% in comparison to the rZOE alone (P = 0.011) and the control medium (P = 0.001).

Conclusion

Since the antimicrobial activities of three different concentrations of AgNPs incorporated into rZOE were equal and AgNPs had lower toxicity at lower concentrations, using AgNPs at 1% concentration is suggested to be mixed with rZOE.

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Title: Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study
Authors: T. S. Shid-Moosavi
N. Mohammadi
Y. Gharamani
M. Motamedifar
A. A. Alizadeh
Publication date: 09-05-2024
Publisher: Springer Berlin Heidelberg
Keyword: Streptococci
Published in: European Archives of Paediatric Dentistry / Issue 3/2024
Print ISSN: 1818-6300
Electronic ISSN: 1996-9805
DOI: https://doi.org/10.1007/s40368-024-00905-7

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Abstract

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