Skip to main content
Top
Published in:

16-01-2024 | Spondyloarthropathy

Understanding Spondyloarthritis Pathogenesis: The Promise of Single-Cell Profiling

Authors: Joerg Ermann, Micah Lefton, Kevin Wei, Maria Gutierrez-Arcelus

Published in: Current Rheumatology Reports | Issue 4/2024

Login to get access

Abstract

Purpose of Review

Single-cell profiling, either in suspension or within the tissue context, is a rapidly evolving field. The purpose of this review is to outline recent advancements and emerging trends with a specific focus on studies in spondyloarthritis.

Recent Findings

The introduction of sequencing-based approaches for the quantification of RNA, protein, or epigenetic modifications at single-cell resolution has provided a major boost to discovery-driven research. Fluorescent flow cytometry, mass cytometry, and image-based cytometry continue to evolve. Spatial transcriptomics and imaging mass cytometry have extended high-dimensional analysis to cells in tissues. Applications in spondyloarthritis include the indexing and functional characterization of cells, discovery of disease-associated cell states, and identification of signatures associated with therapeutic responses. Single-cell TCR-seq has provided evidence for clonal expansion of CD8+ T cells in spondyloarthritis.

Summary

The use of single-cell profiling approaches in spondyloarthritis research is still in its early stages. Challenges include high cost and limited availability of diseased tissue samples. To harness the full potential of the rapidly expanding technical capabilities, large-scale collaborative efforts are imperative.
Literature
5.•
go back to reference Sahir F, Mateo JM, Steinhoff M, Siveen KS. Development of a 43 color panel for the characterization of conventional and unconventional T-cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry. Cytometry A. 2020; https://doi.org/10.1002/cyto.a.24288. Description of a 43-color staining panel for human immune cells documenting the power of spectral flow cytometry. Sahir F, Mateo JM, Steinhoff M, Siveen KS. Development of a 43 color panel for the characterization of conventional and unconventional T-cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate lymphoid cells using spectral flow cytometry. Cytometry A. 2020; https://​doi.​org/​10.​1002/​cyto.​a.​24288. Description of a 43-color staining panel for human immune cells documenting the power of spectral flow cytometry.
8.
30.•
47.
55.••
62.••
go back to reference Yang X, Garner LI, Zvyagin IV, Paley MA, Komech EA, Jude KM, et al. Autoimmunity-associated T cell receptors recognize HLA-B*27-bound peptides. Nature. 2022;612(7941):771–7. https://doi.org/10.1038/s41586-022-05501-7. scTCR-seq analysis of CD8+ T cells from synovial fluid and aqueous humor of HLA-B27+ patients with AS and AAU. Evidence for expansion of distinct TCR clonotypes and identification of HLA-B27 restricted candidate peptides.CrossRefPubMedPubMedCentral Yang X, Garner LI, Zvyagin IV, Paley MA, Komech EA, Jude KM, et al. Autoimmunity-associated T cell receptors recognize HLA-B*27-bound peptides. Nature. 2022;612(7941):771–7. https://​doi.​org/​10.​1038/​s41586-022-05501-7. scTCR-seq analysis of CD8+ T cells from synovial fluid and aqueous humor of HLA-B27+ patients with AS and AAU. Evidence for expansion of distinct TCR clonotypes and identification of HLA-B27 restricted candidate peptides.CrossRefPubMedPubMedCentral
66.••
go back to reference Mehta H, Mashiko S, Angsana J, Rubio M, Hsieh YM, Maari C, et al. Differential changes in inflammatory mononuclear phagocyte and T-cell profiles within psoriatic skin during treatment with guselkumab vs. secukinumab. J Invest Dermatol. 2021;141(7):1707–18 e9. https://doi.org/10.1016/j.jid.2021.01.005. Fluorescent flow cytometric analysis of skin biopsy specimens from a head-to-head randomized controlled clinical trial comparing IL-17A with IL-23p19 inhibition in psoriasis. A differential effect of the two treatment modalities on the inflammatory infiltrate is demonstrated.CrossRefPubMed Mehta H, Mashiko S, Angsana J, Rubio M, Hsieh YM, Maari C, et al. Differential changes in inflammatory mononuclear phagocyte and T-cell profiles within psoriatic skin during treatment with guselkumab vs. secukinumab. J Invest Dermatol. 2021;141(7):1707–18 e9. https://​doi.​org/​10.​1016/​j.​jid.​2021.​01.​005. Fluorescent flow cytometric analysis of skin biopsy specimens from a head-to-head randomized controlled clinical trial comparing IL-17A with IL-23p19 inhibition in psoriasis. A differential effect of the two treatment modalities on the inflammatory infiltrate is demonstrated.CrossRefPubMed
69.••
go back to reference Pachowsky ML, Raimondo MG, Xu C, Rauber S, Tascilar K, Labinsky H, et al. Concise report: a minimal-invasive method to retrieve and identify entheseal tissue from psoriatic arthritis patients. Ann Rheum Dis. 2022; https://doi.org/10.1136/annrheumdis-2021-222061. Description of a novel technique for the isolation of human enthesial cells for cellular and molecular analysis. Pachowsky ML, Raimondo MG, Xu C, Rauber S, Tascilar K, Labinsky H, et al. Concise report: a minimal-invasive method to retrieve and identify entheseal tissue from psoriatic arthritis patients. Ann Rheum Dis. 2022; https://​doi.​org/​10.​1136/​annrheumdis-2021-222061. Description of a novel technique for the isolation of human enthesial cells for cellular and molecular analysis.
72.
go back to reference Rosine N, Rowe H, Koturan S, Yahia-Cherbal H, Leloup C, Watad A, et al. Characterization of blood mucosal-associated invariant T cells in patients with axial spondyloarthritis and of resident mucosal-associated invariant T cells from the axial entheses of non-axial spondyloarthritis control patients. Arthritis Rheumatol. 2022;74(11):1786–95. https://doi.org/10.1002/art.42090.CrossRefPubMedPubMedCentral Rosine N, Rowe H, Koturan S, Yahia-Cherbal H, Leloup C, Watad A, et al. Characterization of blood mucosal-associated invariant T cells in patients with axial spondyloarthritis and of resident mucosal-associated invariant T cells from the axial entheses of non-axial spondyloarthritis control patients. Arthritis Rheumatol. 2022;74(11):1786–95. https://​doi.​org/​10.​1002/​art.​42090.CrossRefPubMedPubMedCentral
75.
Metadata
Title
Understanding Spondyloarthritis Pathogenesis: The Promise of Single-Cell Profiling
Authors
Joerg Ermann
Micah Lefton
Kevin Wei
Maria Gutierrez-Arcelus
Publication date
16-01-2024
Publisher
Springer US
Published in
Current Rheumatology Reports / Issue 4/2024
Print ISSN: 1523-3774
Electronic ISSN: 1534-6307
DOI
https://doi.org/10.1007/s11926-023-01132-7

Other articles of this Issue 4/2024

Current Rheumatology Reports 4/2024 Go to the issue
Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine
Webinar | 06-02-2024 | 20:00 (CET)

Mastering chronic pancreatitis pain: A multidisciplinary approach and practical solutions

Severe pain is the most common symptom of chronic pancreatitis. In this webinar, experts share the latest insights in pain management for chronic pancreatitis patients. Experts from a range of disciplines discuss pertinent cases and provide practical suggestions for use within clinical practice.

Sponsored by: Viatris

Developed by: Springer Healthcare
Live Webinar | 01-10-2024 | 12:30 (CEST)

Recent advances in the use of CAR T-cell therapies in relapsed/refractory diffuse large B-cell lymphoma and follicular lymphoma

Live: Tuesday 1st October 2024, 12:30-14:00 (CEST)

In this live webinar, Professor Martin Dreyling and an esteemed, international panel of CAR-T experts will discuss the very latest data on the safety, efficacy and clinical impact of CAR T-cell therapies in the treatment of r/r DLBCL and r/r FL, as presented at ASH 2023, EU CAR-T 2024, and EHA 2024. 

Please note, this webinar is not intended for healthcare professionals based in the US and UK.

Sponsored by: Novartis Pharma AG

Chaired by: Prof. Martin Dreyling
Developed by: Springer Healthcare