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Open Access 01-12-2024 | Research

Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses

Authors: Chunxia Zhou, Yuqing Hu, Zhuoya Dong, Zheng Wang, Sixie Zheng, Debao Li, Yingying Xiao, Dian Chen, Hao Chen, Sijuan Sun, Lincai Ye, Haibo Zhang

Published in: Journal of Translational Medicine | Issue 1/2024

Abstract

Background

Right ventricular volume overload (RVVO) is one of the most important hemodynamic characteristics in children with congenital heart disease (CHD) and heart failure, and cardiomyocyte (CM) proliferation is one of the most vital factors for improving cardiac performance. However, whether and how RVVO reboots CM proliferation remains elusive.

Methods and results

We first created a neonatal RVVO mouse model via abdominal aorta and inferior vena cava-fistula microsurgery at postnatal day 7 (P7), the edge of CM proliferation window. We subsequently performed bulk RNA-seq, single cell RNA-seq/flow cytometry, and immunofluorescence staining on the right ventricles (RV) of RVVO mice at P14/P21, defined as prepubertal stage, revealing that RVVO temporarily reboots prepubertal CM proliferation via immune responses.

Conclusions

In considering the importance of RVVO and CM proliferation, this study may bring an opportunity to create a novel paradigm to treat pediatric CHDs or heart failure.

Graphical Abstract

Available only for authorised users

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Title: Right ventricular volume overload reboots cardiomyocyte proliferation via immune responses
Authors: Chunxia Zhou
Yuqing Hu
Zhuoya Dong
Zheng Wang
Sixie Zheng
Debao Li
Yingying Xiao
Dian Chen
Hao Chen
Sijuan Sun
Lincai Ye
Haibo Zhang
Publication date: 01-12-2024
Publisher: BioMed Central
Published in: Journal of Translational Medicine / Issue 1/2024
Electronic ISSN: 1479-5876
DOI: https://doi.org/10.1186/s12967-024-05839-8

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