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Acta Neuropathologica Communications
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Open Access 01-12-2024 | Research

Retinal cytoarchitecture is preserved in an organotypic perfused human and porcine eye model

Authors: Darren Chan, Jenny Wanyu Zhang, Gah-Jone Won, Jeremy M. Sivak

Published in: Acta Neuropathologica Communications | Issue 1/2024

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Abstract

Pathobiology of the intact human retina has been challenging to study due to its relative inaccessibility and limited sample availability. Thus, there is a great need for new translational models that can maintain human retinal integrity and cytoarchitecture. The role of physiologic intraocular pressure (IOP) and fluid flow on retinal tissue has not been well studied. Here, we present an ex vivo organotypic model to assess the impact of physiological intraocular perfusion on retinal cytoarchitecture and cell survival. We demonstrate that retinal cytoarchitecture is remarkably well preserved following re-establishment of physiological IOP and aqueous humor dynamics for up to 24 h in ex vivo whole globe porcine and human eyes, comparable to freshly preserved control eyes. Accordingly, cell death was minimized in the perfused retinas, which also displayed normal markers of cellular metabolism and astrogliosis. These results are in marked contrast to contralateral control eyes without active perfusion, which displayed excessive cell death and disrupted cytoarchitecture at the same time point. These experiments demonstrate the critical impact that physiological pressure and fluid flow have on retinal tissue, and introduce a new pre-clinical model to study human and porcine retinal health and degeneration in a relevant biomechanical setting.
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Metadata
Title
Retinal cytoarchitecture is preserved in an organotypic perfused human and porcine eye model
Authors
Darren Chan
Jenny Wanyu Zhang
Gah-Jone Won
Jeremy M. Sivak
Publication date
01-12-2024
Publisher
BioMed Central
Published in
Acta Neuropathologica Communications / Issue 1/2024
Electronic ISSN: 2051-5960
DOI
https://doi.org/10.1186/s40478-024-01892-y

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