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26-12-2023 | Respiratory Microbiota | Original Contribution

The anti-hyperuricemic and gut microbiota regulatory effects of a novel purine assimilatory strain, Lactiplantibacillus plantarum X7022

Authors: Yuan Zou, Kum-Song Ro, Chentian Jiang, Deyi Yin, Li Zhao, Daihui Zhang, Lei Du, Jingli Xie

Published in: European Journal of Nutrition | Issue 3/2024

Abstract

Purpose

Probiotics have been reported to effectively alleviate hyperuricemia and regulate the gut microbiota. The aim of this work was to study the in vivo anti-hyperuricemic properties and the mechanism of a novel strain, Lactiplantibacillus plantarum X7022.

Methods

Purine content and mRNA expression of purine assimilation related enzymes were determined by HPLC and qPCR, respectively. Hyperuricemic mice were induced by potassium oxonate and hypoxanthine. Uric acid (UA), blood urea nitrogen, creatinine and renal inflammation were examined by kits. The expression of renal UA transporters was subjected to western blotting. Kidney tissues were sectioned for histological analysis. The fecal short-chain fatty acids (SCFAs) were determined by HPLC, and gut microbiota was investigated using the 16S rDNA metagenomic sequencing.

Results

L. plantarum X7022 possesses a complete purine assimilation pathway and can exhaust xanthine, guanine, and adenine by 82.1%, 33.1%, and 12.6%, respectively. The strain exhibited gastrointestinal viability as 44% at the dose of 10⁹ CFU/mL in mice. After four-week administration of the strain, a significant decrease of 35.5% in the serum UA level in hyperuricemic mice was achieved. The diminished contents of fecal propionate and butyrate were dramatically boosted. The treatment also alleviated renal inflammation and restored renal damage. The above physiological changes may due to the inhibited xanthine oxidase (XO) activity, as well as the expressional regulation of UA transporters (GLUT9, URAT1 and OAT1) to the normal level. Notably, gut microbiota dysbiosis in hyperuricemic mice was improved with the inflammation and hyperuricemia related flora depressed, and SCFAs production related flora promoted.

Conclusion

The strain is a promising probiotic strain for ameliorating hyperuricemia.

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Title: The anti-hyperuricemic and gut microbiota regulatory effects of a novel purine assimilatory strain, Lactiplantibacillus plantarum X7022
Authors: Yuan Zou
Kum-Song Ro
Chentian Jiang
Deyi Yin
Li Zhao
Daihui Zhang
Lei Du
Jingli Xie
Publication date: 26-12-2023
Publisher: Springer Berlin Heidelberg
Keywords: Respiratory Microbiota
Xanthine Alkaloid
Probiotics
Allopurinol
Published in: European Journal of Nutrition / Issue 3/2024
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-023-03291-w

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Abstract

Purpose

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Conclusion

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