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European Journal of Clinical Microbiology & Infectious Diseases

20-11-2023 | Brief Report

Rapid detection of S. pyogenes and S. pneumoniae in pleural fluid for diagnosis of parapneumonic empyema

Authors: Kelly De Schuyter, Jerina Boelens, Anne-Sophie Messiaen, Petra Schelstraete, Bruno Verhasselt, Diana Huis In’t Veld, Steven Callens, Erica Sermijn, Yannick Vande Weygaerde, Stien Vandendriesche

Published in: European Journal of Clinical Microbiology & Infectious Diseases

Abstract

The aim of this study was to assess the reliability of rapid antigen detection tests (RADT) for Streptococcus pyogenes (GAS) and Streptococcus pneumoniae on pleural fluid samples for diagnosis of parapneumonic effusion/empyema (PPE) and their potential for improving pathogen identification rates. Sixty-three pleural samples were included from 54 patients on which GAS and S. pneumoniae RADT (BinaxNOW), culture, 16S rRNA PCR, and S. pneumoniae–specific PCR were performed. GAS RADT showed a sensitivity of 95.2% and a specificity of 100%. Pneumococcal RADT showed a sensitivity of 100% and specificity of 88.6%. Both RADT increased the pathogen identification rate in PPE compared to culture.

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Title: Rapid detection of S. pyogenes and S. pneumoniae in pleural fluid for diagnosis of parapneumonic empyema
Authors: Kelly De Schuyter
Jerina Boelens
Anne-Sophie Messiaen
Petra Schelstraete
Bruno Verhasselt
Diana Huis In’t Veld
Steven Callens
Erica Sermijn
Yannick Vande Weygaerde
Stien Vandendriesche
Publication date: 20-11-2023
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Clinical Microbiology & Infectious Diseases
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-023-04710-w

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