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Open Access 01-12-2023 | Pseudomonas Aeruginosa | Research

Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits

Authors: Zeinab A. Saad, Hazem Elnashar, Sahar Negm, Hala A. Elsayed, Mohamed Gaber Abdallah, Tamer M. M. Abuamara, Wagih M. Abd-Elhay, Hanan M. Elghonemy

Published in: BMC Ophthalmology | Issue 1/2023

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Abstract

Background and purpose

Collagen cross-linking (CXL) has evolved as an essential therapeutic approach for corneal infections, allowing for rapidly eliminating the infecting microorganism while reducing inflammation. This study aims to evaluate the efficacy of CXL as a monotherapy for managing infectious keratitis caused by Fusarium solani and Pseudomonas aeruginosa.

Materials and methods

Forty-eight white New Zealand rabbits weighing approximately 1.5–2 KG were included. The cornea of one eye of each rabbit was inoculated with either Fusarium solani or Pseudomonas aeruginosa. Group A served as a control and was subdivided into two subgroups, A1 and A2; each subgroup consisted of 8 eyes and was injected with either Fusarium solani or Pseudomonas aeruginosa, respectively. Group B (16 eyes) was inoculated with Fusarium solani, while group C (16 eyes) were inoculated with Pseudomonas aeruginosa. All animals in Group B and C received CXL treatment one week after inoculation of the organisms and after corneal abscess formation was confirmed. At the same time, animals in Group A were left untreated.

Results

There was a statistically significant reduction in the number of colony-forming units (CFU) in Group B following CXL. No growth existed in any samples at the end of the 4th week. There was a statistically significant difference in the number of CFU between group B and the control group (p < 0.001). In group C, there was a statistically significant reduction in the CFU at the end of the first week after CXL. However, there was regrowth in all samples afterward. All 16 models in Group C showed uncountable and extensive growth during the subsequent follow-ups. There was no statistically significant difference between the number of CFU in Group C and the control group. Histopathology showed lesser corneal melting in CXL-treated Pseudomonas aeruginosa.

Conclusions

Collagen cross-linking is promising monotherapy and alternative treatment in managing infective keratitis caused by Fusarium solani but is less effective in Pseudomonas aeruginosa as monotherapy.
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Metadata
Title
Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits
Authors
Zeinab A. Saad
Hazem Elnashar
Sahar Negm
Hala A. Elsayed
Mohamed Gaber Abdallah
Tamer M. M. Abuamara
Wagih M. Abd-Elhay
Hanan M. Elghonemy
Publication date
01-12-2023
Publisher
BioMed Central
Published in
BMC Ophthalmology / Issue 1/2023
Electronic ISSN: 1471-2415
DOI
https://doi.org/10.1186/s12886-023-03007-y

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