Skip to main content
Top
Published in: Journal of Natural Medicines 3/2024

25-04-2024 | Original Paper

Promotion of neurite outgrowth by 3,5,7,3ʹ,4ʹ-pentamethoxyflavone is mediated through ERK signaling pathway in Neuro2a cells

Authors: Shin-Ji Park, So-Young An, Yeon Jin An, Kyoung-Sook Kim, Hyunju Kim, Jong Hyun Cho, Young-Choon Lee

Published in: Journal of Natural Medicines | Issue 3/2024

Login to get access

Abstract

In this study, the effects of 3,5,7,3ʹ,4ʹ-pentamethoxyflavone (KP1), a major bioactive ingredient isolated from the Kaempferia parviflora rhizomes, on a neurite outgrowth in Neuro2a cells and its mechanism have been investigated. KP1 increased concentration-dependently the percentage of neurite-bearing cells. KP1 showed a remarkable capability to elicit neurite outgrowth in Neuro2a cells, as evidenced by morphological alterations and immunostaining using anti-class III β-tubulin and anti-NeuN antibodies. KP1 also displayed a higher neurogenic activity than retinoic acid (RA), a promoter of neurite outgrowth in Neuro2a cells. KP1 treatment caused significant elevation in phosphorylation of extracellular signal-regulated kinase (ERK), p38 mitogen-activated protein kinase (p38 MAPK) and glycogen synthase kinase-3β (GSK-3β). However, KP1-triggered neurite outgrowth was markedly inhibited by treatment with the ERK inhibitor U0126, whereas p38 MAPK inhibitor SB203580 and GSK-3β inhibitor SB216763 did not influence KP1-induced neurite outgrowth. These results demonstrate that KP1 elicits neurite outgrowth and triggers cell differentiation of Neuro2a cells through ERK signal pathway.

Graphical abstract

Appendix
Available only for authorised users
Literature
24.
go back to reference Seo SH, Lee YC, Moon HI (2017) Acetyl-cholinesterase inhibitory activity of methoxyflavones isolated from Kaempferia parviflora. Nat Prod Commun 12:21–22PubMed Seo SH, Lee YC, Moon HI (2017) Acetyl-cholinesterase inhibitory activity of methoxyflavones isolated from Kaempferia parviflora. Nat Prod Commun 12:21–22PubMed
Metadata
Title
Promotion of neurite outgrowth by 3,5,7,3ʹ,4ʹ-pentamethoxyflavone is mediated through ERK signaling pathway in Neuro2a cells
Authors
Shin-Ji Park
So-Young An
Yeon Jin An
Kyoung-Sook Kim
Hyunju Kim
Jong Hyun Cho
Young-Choon Lee
Publication date
25-04-2024
Publisher
Springer Nature Singapore
Published in
Journal of Natural Medicines / Issue 3/2024
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI
https://doi.org/10.1007/s11418-024-01809-y

Other articles of this Issue 3/2024

Journal of Natural Medicines 3/2024 Go to the issue