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Planta Med 2003; 69(12): 1125-1129
DOI: 10.1055/s-2003-45194
Original Paper
Biochemistry
© Georg Thieme Verlag Stuttgart · New York

Effects of Selected Flavonoids and Caffeic Acid Derivatives on Hypoxanthine-Xanthine Oxidase-Induced Toxicity in Cultivated Human Cells

Gabriele Beyer1 , Matthias F. Melzig2
  • 1Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany
  • 2Institut für Pharmazie, Freie Universität Berlin, Berlin, Germany
Further Information

Publication History

Received: May 23, 2003

Accepted: September 7, 2003

Publication Date:
29 January 2004 (online)

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Abstract

Tissue damage as a result of oxygen radical generation may be involved in the pathogenesis of different diseases, carcinogenesis, aging and cell death. The inhibition of the proliferation rate of the immortalised human cell line ECV 304 after oxidant damage by oxygen radicals generated in a hypoxanthine-xanthine oxidase system and the protection provided by some selected flavone and flavonol glycosides as well as by caffeic acid and its derivatives was determined. The cytotoxicity of the reactive oxygen species was differentially influenced by selected flavonoids and seems to be determined by the pattern of substitution and by their lipophilicity. Apigenin and quercetin demonstrated the strongest effect on the inhibition of hypoxanthine-xanthine oxidase-induced toxicity (50 % restitution of the cells at a concentration of 0.36 μM and 3.1 μM, respectively). The beneficial effect of the flavonol glycosides rutin and hyperoside was weak, whereas flavone glycosides such as diosmin showed a better effect of protection.

Key words

ECV 304-cells - Hypoxanthine - xanthine oxidase - radical scavenger activity - flavonols - flavones - caffeic acid derivatives

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Prof. M. F. Melzig

Institut für Pharmazie

Freie Universität Berlin

Königin-Luise-Str. 2

14195 Berlin

Germany

Phone: +49-30-838-51451

Fax: +49-30-838-51461

Email: melzig@zedat.fu-berlin.de

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