Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation
- Open Access
- 12-01-2024
- Postmenopause
- Original Contribution
Published in:
- Authors
- Mary F. O’Leary
- Sarah R. Jackman
- Joanna L. Bowtell
- Published in
- European Journal of Nutrition | Issue 3/2024
Abstract
Purpose
Shatavari is an understudied, widely available herbal supplement. It contains steroidal saponins and phytoestrogens. We previously showed that six weeks of shatavari supplementation improved handgrip strength and increased markers of myosin contractile function. Mechanistic insights into shatavari’s actions are limited. Therefore, we performed proteomics on vastus lateralis (VL) samples that remained from our original study.
Methods
In a randomised double-blind trial, women (68.5 ± 6 years) ingested either placebo or shatavari (equivalent to 26,500 mg/d fresh weight) for six weeks. Tandem mass tag global proteomic analysis of VL samples was conducted (N = 7 shatavari, N = 5 placebo). Data were normalized to total peptides and scaled using a reference sample. Data were filtered using a 5% FDR. For each protein, the pre to post supplementation difference was expressed as log2 fold change. Welch’s t tests with Benjamini–Hochberg corrections were performed for each protein. Pathway enrichment (PADOG, CAMERA) was interrogated in Reactome (v85).
Results
No individual protein was significantly different between supplementation conditions. Both PADOG and CAMERA indicated that pathways related to (1) Integrin/MAPK signalling, (2) metabolism/insulin secretion; (3) cell proliferation/senescence/DNA repair/cell death; (4) haemostasis/platelets/fibrin; (5) signal transduction; (6) neutrophil degranulation and (7) chemical synapse function were significantly upregulated. CAMERA indicated pathways related to translation/amino acid metabolism, viral infection, and muscle contraction were downregulated.
Conclusion
Our analyses indicate that shatavari may support muscle adaptation responses to exercise. These data provide useful signposts for future investigation of shatavari’s utility in conserving and enhancing musculoskeletal function in older age.
Trial registration
NCT05025917 30/08/21, retrospectively registered.
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- Title
- Shatavari supplementation in postmenopausal women alters the skeletal muscle proteome and pathways involved in training adaptation
- Authors
-
Mary F. O’Leary
Sarah R. Jackman
Joanna L. Bowtell
- Publication date
- 12-01-2024
- Publisher
- Springer Berlin Heidelberg
- Keyword
- Postmenopause
- Published in
-
European Journal of Nutrition / Issue 3/2024
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215 - DOI
- https://doi.org/10.1007/s00394-023-03310-w
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