Published in:
Open Access 22-09-2023 | Polymerase Chain Reaction | Gynecologic Oncology
Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
Published in: Archives of Gynecology and Obstetrics | Issue 4/2024
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Purpose
In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ).
Methods
MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level.
Results
The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ.
Conclusion
MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level.