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26-07-2023 | Polycystic Ovary Syndrome | Original Article

Improves immunological and histological alterations associated with Polycystic ovarian syndrome in DHEA -induced mice by Licorice extract

Authors: Ensieh Seif Amirhoseiny, Ali Ganji, Ghasem Mosayebi, Maryam Shamsi, Ali Ghazavi

Published in: Comparative Clinical Pathology | Issue 5/2023

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Abstract

Polycystic ovary syndrome (PCOS) is a complex syndrome that causes infertility in women. Licorice is one of the medicinal plants with antioxidant properties used to treat diseases. This study investigated the anti-inflammatory effect of licorice extract on PCOS. After induction of PCOS by subcutaneous injection (SC) of dehydroepiandrosterone (DHEA) (60 mg/kg) in BALB/C mice for 21 days, the mice were treated with licorice extract (50 mg/kg) for 21 days. At the end of experimental procedures, ovarian morphology, blood antioxidant capacity, nitric oxide (NO), the abundance of Treg cells, and expression of matrix metalloproteinase-9 (MMP-9), transforming growth factor-ß (TGF-ß), cyclooxygenase-2 genes (COX-2), and tumor necrosis factor-alpha (TNF-α) were measured. The estrus cycle returned to normal after treatment with licorice extract. It was observed that the licorice number of ovarian cysts was significantly higher in the licorice group than in the PCOS group, and the corpus luteum was lower than in the control group. NO, FRAP, and Treg cell numbers did not significantly alter. The TGF- gene’s expression level significantly increased, but the expression level of TNF-α, COX-2, and MMP-9 did not change significantly. Immune responses play an important role in the development of inflammation. Licorice extract, with anti-inflammatory properties, may be an effective supplement in improving the histological and immunological changes of PCOS.
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Metadata
Title
Improves immunological and histological alterations associated with Polycystic ovarian syndrome in DHEA -induced mice by Licorice extract
Authors
Ensieh Seif Amirhoseiny
Ali Ganji
Ghasem Mosayebi
Maryam Shamsi
Ali Ghazavi
Publication date
26-07-2023
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 5/2023
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-023-03492-z

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