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26-12-2023 | Myocardial Infarction | Sleep Breathing Physiology and Disorders • Original Article

Guanxin V alleviates ventricular remodeling after acute myocardial infarction with circadian disruption by regulating mitochondrial dynamics

Authors: Songyi Cheng, Jing Wu, Yinghao Pei, Huaqin Tong, Manlu Fan, Qian Xiang, Yuhan Ding, Liang Xie, Haowen Zhang, Weixin Sun, Xiaoxiao Zhang, Yongchun Zhu, Ning Gu

Published in: Sleep and Breathing | Issue 2/2024

Abstract

Purpose

Circadian disruption has been a common issue due to modern lifestyles. Ventricular remodeling (VR) is a pivotal progressive pathologic change after acute myocardial infarction (AMI) and circadian disruption may have a negative influence on VR according to the latest research. Whether or not Guanxin V (GXV) has a positive effect on VR after AMI with circadian disruption drew our interest.

Methods

Rats were randomly divided into a sham group, an AMI group, an AMI with circadian disruption group, and an AMI with circadian disruption treated with the GXV group according to a random number table. RNA sequencing (RNA-Seq) was utilized to confirm the different expressed genes regulated by circadian disruption. Cardiac function, inflammation factors, pathological evaluation, and mitochondrial dynamics after the intervention were conducted to reveal the mechanism by which GXV regulated VR after AMI with circadian disruption.

Results

RNA-Seq demonstrated that NF-κB was up-regulated by circadian disruption in rats with AMI. Functional and pathological evaluation indicated that compared with the AMI group, circadian disruption was associcataed with deteriorated cardiac function, expanded infarcted size, and exacerbated fibrosis and cardiomyocyte apoptosis. Further investigation demonstrated that mitochondrial dynamics imbalance was induced by circadian disruption. GXV intervention reversed the inflammatory status including down-regulation of NF-κB. Reserved cardiac function, limited infarct size, and ameliorated fibrosis and apoptosis were also observed in the GXV treated group. GXV maintained mitochondrial fission/fusion imbalance through suppressed expression of mitochondrial fission–associated proteins.

Conclusion

The study findings suggest that identified mitochondrial dysfunctions may underlie the link between circadian disruption and VR. GXV may exert cardioprotection after AMI with circadian disruption through regulating mitochondrial dynamics.

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Title: Guanxin V alleviates ventricular remodeling after acute myocardial infarction with circadian disruption by regulating mitochondrial dynamics
Authors: Songyi Cheng
Jing Wu
Yinghao Pei
Huaqin Tong
Manlu Fan
Qian Xiang
Yuhan Ding
Liang Xie
Haowen Zhang
Weixin Sun
Xiaoxiao Zhang
Yongchun Zhu
Ning Gu
Publication date: 26-12-2023
Publisher: Springer International Publishing
Keywords: Myocardial Infarction
CSF Drainage
Published in: Sleep and Breathing / Issue 2/2024
Print ISSN: 1520-9512
Electronic ISSN: 1522-1709
DOI: https://doi.org/10.1007/s11325-023-02974-2

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Guanxin V alleviates ventricular remodeling after acute myocardial infarction with circadian disruption by regulating mitochondrial dynamics

Abstract

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Conclusion

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