13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

- Oral Presentation 101

TITLE: Effects of laser phototherapy and growth factors PDGF and BMP-2 on the odontogenic differentiation of dental pulp stem cells.

AUTHORS: Ferreira LS, Cai S, Barros JA, Patel SA, Chen W, Marques MM.

University of Sao Paulo, Ibirapuera Universtiy, University of Texas

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S101.

* doi:10.4317/medoral.17643600

http://dx.doi.org/10.4317/medoral.17643600

Abstract

Laser phototherapy (LPT) is able to increase cellular metabolism which in turn could influence the differentiation of human dental pulp stem cells (hDPSCs). PDGF and BMP2 are growth factors associated with cell differentiation in mineralized tissues such as bone and dentin. The aim of this study was to analyze the effects of LPT, PDGF and BMP2 combined or not on the odontogenic differentiation of hDPSCs. Cells were grown in regular medium (G1) and LPT (G2), mineralizing medium-MM (G3) and LPT (G4), MM containing PDGF (G5) and LPT (G6), MM containing BMP2 (G7) and LPT (G8). LPT was performed in punctual and contact mode with a semiconductor diode laser (660nm, InGaAlP, beam area of 0.028cm²) using the following parameters: 20mW, 5J/cm² and 7s p/point (0.14J p/point). Differentiation was assessed by expression of DSPP,DMP1 and OCN genes; alkaline phosphatase (ALP) activity and calcium deposition in 3, 7 and 14 days. Data were compared by ANOVA and Tukey´s test (p<0.05). Expression of DSPP, DMP1 and OCN genes were significantly higher in G8 in 14 days compared to all other groups, except for G3 and G7. G8 showed the highest ALP activity in 3 days and the largest amount of calcium deposits in 14 days. The most striking feature on odontogenic differentiation induction of hDPSCs was observed when LPT was associated with BMP2. Therefore, the use of this combined therapy could be of rele-vance for tissue engineering and for recovery of dental pulp physiology in cases of exposure.