13th World Congress for Laser Dentistry

26-28, April 2012

Barcelona, Spain

Meeting Abstract

Sponsors: World Federation for Laser Dentistry

- Oral Presentation 100

TITLE: Effect of laser phototherapy on gene expression of stem cell markers of human dental pulp stem cells. .

AUTHORS: Ferreira LS, Destro MFSS, Maranduba CMC, Miyagi SPH, Marques MM.

University of Sao Paulo, Ibirapuera University, Federal University of Juiz de Fora, University of Braz Cubas

SOURCE: Med Oral Patol Oral Cir Bucal. 2012 May 1;17(Supplement1):S100.

* doi:10.4317/medoral.17643599

http://dx.doi.org/10.4317/medoral.17643599

Abstract

The potential of using laser phototherapy (LPT) to improve the performance of human dental pulp stem cells (hDPSCs) in tissue engineering is large. However little is know about laser effects on the patterns of stem cell markers in these cells. The objective of this study was to analyze the effects of LPT on gene expression of embryonic and mesenchymal stem cell markers of hDPSCs from deciduous teeth grown in nutritional deficit condition. hDPSCs grown in culture medium supplemented with 15% or 2% fetal bovine serum (nutritional deficit) were irradiated or not with a semicondutor diode laser (660nm, InGaAlP, spot area of 0,028cm²) using the following parameters: 20mW, 0.714W/cm², 5 or 20J/cm², 7 or 28s and 0.14J or 0.56J per point, respectively. After 48h, gene expression of embryonic and mesenchymal stem cell markers Oct-4, Nestin, CD90 and CD105 were assessed by quantitative real time PCR assay. The data were statistically compared by ANOVA and complemented by Tukey’s test (p≤0.05). LPT significantly increased Nestin and CD90 gene expression for both energy densities used: 5 and 20J/cm². For Oct-4 and CD105 genes LPT had no effect in any of the parameters tested. LPT is able to stimulate expression of stem cell markers gene profile of hDPSCs in certain irradiation parameters. This data should be taken in consideration when hDPSCs under stress conditions undergo LPT for tissue engineering and cell therapy purposes.