Food Restriction Causes Low Bone Strength and Microarchitectural Deterioration in Exercised Growing Male Rats

Satoshi HATTORI; Jong-Hoon PARK; Umon AGATA; Masaya ODA; Michito HIGANO; Yuki AIKAWA; Takayuki AKIMOTO; Yoshiharu NABEKURA; Hideyuki YAMATO; Ikuko EZAWA; Naomi OMI

doi:10.3177/jnsv.60.35

Regular Paper

Food Restriction Causes Low Bone Strength and Microarchitectural Deterioration in Exercised Growing Male Rats

Satoshi HATTORI, Jong-Hoon PARK, Umon AGATA, Masaya ODA, Michito HIGANO, Yuki AIKAWA, Takayuki AKIMOTO, Yoshiharu NABEKURA, Hideyuki YAMATO, Ikuko EZAWA, Naomi OMI

Author information

Satoshi HATTORI
Comprehensive Human Sciences, University of Tsukuba
Jong-Hoon PARK
Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo
Department of Physical Education, Konkuk University
Umon AGATA
Comprehensive Human Sciences, University of Tsukuba
Masaya ODA
Comprehensive Human Sciences, University of Tsukuba
Michito HIGANO
Comprehensive Human Sciences, University of Tsukuba
Yuki AIKAWA
Comprehensive Human Sciences, University of Tsukuba
Takayuki AKIMOTO
Division of Regenerative Medical Engineering, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, University of Tokyo
Yoshiharu NABEKURA
Comprehensive Human Sciences, University of Tsukuba
Faculty of Health and Sport Sciences, University of Tsukuba
Hideyuki YAMATO
Biomedical Research Laboratories, Kureha Co., Ltd.
Ikuko EZAWA
Department of Food and Nutrition, Japan Women’s University
Naomi OMI
Comprehensive Human Sciences, University of Tsukuba
Faculty of Health and Sport Sciences, University of Tsukuba

Keywords: low energy availability, voluntary running exercise, bone strength, bone morphology, bone mineral density

JOURNAL FREE ACCESS

2014 Volume 60 Issue 1 Pages 35-42

DOI https://doi.org/10.3177/jnsv.60.35

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Abstract

The pathogenesis of bone disorders in young male athletes has not been well understood. We hypothesized that bone fragility is caused by low energy availability, due to insufficient food intake and excessive exercise energy expenditure in young male athletes. To examine this hypothesis, we investigated the influence of food restriction on bone strength and bone morphology in exercised growing male rats, using three-point bending test, dual-energy X-ray absormetry, and micro-computed tomography. Four-week-old male Sprague-Dawley rats were divided randomly into the following groups: the control (Con) group, exercise (Ex) group, food restriction (R) group, and food restriction plus exercise (REx) group after a 1-wk acclimatization period. Thirty-percent food restriction in the R and REx groups was carried out in comparison with that in the Con group. Voluntary running exercise was performed in the Ex and REx groups. The experimental period lasted 13 wk. At the endpoint of this experiment, the bone strength of the femurs and tibial BMD in the REx group were significantly lower than those in the Con group. Moreover, trabecular bone volume and cortical bone volume in the REx group were also significantly lower than those in the Con group. These findings indicate that food restriction causes low bone strength and microarchitectural deterioration in exercised growing male rats.

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