The influence of honey on biochemical and biophysical parameters of wounds was assessed by making an excision wound by cutting away a 4cm² (2cm×2cm) full-thickness patch of skin from the shaven back of rats under ether anaesthesia. One milliliter of pure, commercial, unboiled honey was applied topically (Group II), or administered orally (Group III) or intraperitoneally (Group IV). The control rats were left untreated (Group I). The rate of healing was assessed by estimation of some biochemical parameters like DNA, total protein, total collagen, hexosamine and uronic acid contents of the granulation tissues formed different times after wound creation. All the biochemical parameters increased in honey-treated animals when compared with the control values. The increases were very significant with oral and intraperitoneal treatment when compared with those with the topical. Biophysical parameters like tensile strength, stress-strain behavior, rate of contraction, and period of epithelialization were also studied. There was an approximately 21% increase in tensile strength in Group II rats, whereas the increase was more in Group III (34.5%) and Group IV (52%) rats. The stress-strain behavior also followed the same trend. A significant increase in the rate of contraction was observed equally in all three honey-treated groups when their values were compared to the control. Among the three experimental groups, the intraperitoneal treatment decreased the period of epithelialization more significantly (37%) than the oral (22%) and topical (15%) treatments. The decrease in the period of epithelialization showed that the time taken by the wounds to heal was less with systemic treatment than with no treatment (control).