A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed          ICR mice

Zhang Zhuhua; Wang Zhiquan; Yang Zhen; Niu Yixin; Zhang Weiwei; Li Xiaoyong; Liu Yueming; Zhang Hongmei; Qin Li; Su Qing

doi:10.1538/expanim.14-0086

Originals

A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice

Zhang Zhuhua, Wang Zhiquan, Yang Zhen, Niu Yixin, Zhang Weiwei, Li Xiaoyong, Liu Yueming, Zhang Hongmei, Qin Li, Su Qing

Author information

Zhang Zhuhua
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Wang Zhiquan
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Yang Zhen
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Niu Yixin
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Zhang Weiwei
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Li Xiaoyong
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Liu Yueming
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Zhang Hongmei
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Qin Li
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China
Su Qing
Department of Endocrinology, Xinhua hospital, Shanghai Jiaotong University School of Medicine, 1665 Kongjiang Road, Shanghai, P.R.China

Keywords: animal model, high-fat-high-fructose, Institute of Cancer Research mice, insulin resistance, metabolic syndrome

JOURNAL FREE ACCESS

2015 Volume 64 Issue 4 Pages 435-442

DOI https://doi.org/10.1538/expanim.14-0086

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Abstract

Currently, the metabolic syndrome (MS) is occurring at growing rates worldwide, raising extensive concerns on the mechanisms and therapeutic interventions for this disorder. Herein, we described a novel method of establishing MS model in rodents. Male Institute of Cancer Research (ICR) mice were fed with high-fat-high-fructose (HFHF) diet or normal chow (NC) respectively for 12 weeks. Metabolic phenotypes were assessed by glucose tolerance test, insulin tolerance test and hyperinsulinemic-euglycemic clamp. Blood pressure was measured by a tail-cuff system. At the end of the experiment, mice were sacrificed, and blood and tissues were harvested for subsequent analysis. Serum insulin levels were measured by ELISA, and lipid profiles were determined biochemically. The HFHF diet-fed ICR mice exhibited obvious characteristics of the components of MS, including obvious obesity, severe insulin resistance, hyperinsulinemia, dislipidemia, significant hypertension and hyperuricemia. Our data suggest that HFHF diet-fed ICR mice may be a robust and efficient animal model that could well mimic the basic pathogenesis of human MS.

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