Three quercetin glycosides, designated f1, f3, and f5, were obtained from the fruits of Rosa multiflora THUNB. The structures of f1 and f3, new flavonol glycosides, were determined to be quercetin 3-β-D-glucopyranosyl-(1→4)-α-L-ramnopyranoside (I) and quercetin 3-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→4)-α-L-ramnopyranoside (II), respectively, by means of acidic hydrolysis, enzymatic hydrolysis, hydrolysis of methylates, nuclear magnetic resonance measurement of trimethylsilyl ether and methanolysis of fully methylated compounds. f5 was identified with quercitrin (III).