New kaempferol glycosides, named multiflorin-A and -B, were isolated from the active fraction of a purgative drug, Rosa multiflora THUNB., together with kaempferol 3-α-L-rhamnopyranoside (III) and methyl gallate. Multiflorin-A and -B were determined to be kaempferol, 3-β-D-glucopyranosyl-(1→4)-α-L-rhamnopyranoside monoacetate (II) and kaempferol 3-β-D-glucopyranosyl (1→4)-α-L-rhamnopyranoside (I), respectively. Among these constituents, multiflorin-A has the strongest purgative activity.