Brazilian Natural Medicines. IV.  New Noroleanane-Type Triterpene and Ecdysterone-Type Sterol Glycosides and Melanogenesis Inhibitors from the Roots of Pfaffia glomerata

Seikou Nakamura; Gang Chen; Souichi Nakashima; Hisashi Matsuda; Yuehu Pei; Masayuki Yoshikawa

doi:10.1248/cpb.58.690

Regular Articles

Brazilian Natural Medicines. IV. New Noroleanane-Type Triterpene and Ecdysterone-Type Sterol Glycosides and Melanogenesis Inhibitors from the Roots of Pfaffia glomerata

Seikou Nakamura, Gang Chen, Souichi Nakashima, Hisashi Matsuda, Yuehu Pei, Masayuki Yoshikawa

Author information

Keywords: Pfaffia glomerata, Brazil ginseng, pfaffiaglycoside, noroleanane-type triterpene, ecdysterone-type sterol glycoside, melanogenesis inhibitor

JOURNAL FREE ACCESS

2010 Volume 58 Issue 5 Pages 690-695

DOI https://doi.org/10.1248/cpb.58.690

Details

Abstract

The ethyl acetate and 1-butanol soluble fractions of the roots of Pfaffia glomerata were found to show inhibitory effects on melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells. From the ethyl acetate and 1-butanol soluble fractions, we isolated a new noroleanane-type triterpene, pfaffianol A, its glycosides, pfaffiaglycosides A and B, and ecdysterone-type sterol glycosides, pfaffiaglycosides C, D, and E, together with eight known constituents. The structures of new constituents were determined on the basis of physicochemical and chemical evidence. Among them, pfaffianol A (IC₅₀=44 μM) and pfaffoside C (IC₅₀=92 μM) substantially inhibited melanogenesis without cytotoxic effects. The inhibitory effects were stronger than that of reference compound, arbutin (IC₅₀=174 μM).

Corresponding author

Register with J-STAGE for free!