The Effects of the Selective Serotonin Reuptake Inhibitor Fluvoxamine on Voltage-Dependent K+ Channels in Rabbit Coronary Arterial Smooth Muscle Cells

Da Hye Hong; Hongliang Li; Han Sol Kim; Hye Won Kim; Sung Eun Shin; Won-Kyo Jung; Sung Hun Na; Il-Whan Choi; Amy Leanne Firth; Won Sun Park; Dae-Joong Kim

doi:10.1248/bpb.b15-00207

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The Effects of the Selective Serotonin Reuptake Inhibitor Fluvoxamine on Voltage-Dependent K⁺ Channels in Rabbit Coronary Arterial Smooth Muscle Cells

Da Hye Hong, Hongliang Li, Han Sol Kim, Hye Won Kim, Sung Eun Shin, Won-Kyo Jung, Sung Hun Na, Il-Whan Choi, Amy Leanne Firth, Won Sun Park, Dae-Joong Kim

Author information

Da Hye Hong
Department of Physiology, Kangwon National University School of Medicine
Hongliang Li
Department of Physiology, Kangwon National University School of Medicine
Han Sol Kim
Department of Physiology, Kangwon National University School of Medicine
Hye Won Kim
Department of Physiology, Kangwon National University School of Medicine
Sung Eun Shin
Department of Physiology, Kangwon National University School of Medicine
Won-Kyo Jung
Department of Biomedical Engineering, Center for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University
Sung Hun Na
Department of Obstetrics and Gynecology, Kangwon National University Hospital, School of Medicine, Kangwon National University
Il-Whan Choi
Department of Microbiology, Inje University College of Medicine
Amy Leanne Firth
Laboratory of Genetics, Salk Institute for Biological Studies
Won Sun Park
Department of Physiology, Kangwon National University School of Medicine
Dae-Joong Kim Corresponding author
Department of Anatomy and Cell Biology, Kangwon National University School of Medicine

Keywords: fluvoxamine, voltage-dependent K⁺ channel, coronary artery, smooth muscle, selective serotonin reuptake inhibitor

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Supplementary material

2015 Volume 38 Issue 8 Pages 1208-1213

DOI https://doi.org/10.1248/bpb.b15-00207

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Abstract

We demonstrated the inhibitory effect of fluvoxamine, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent K⁺ (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells using a whole-cell patch clamp technique. Fluvoxamine reduced the amplitude of Kv currents in a concentration-dependent manner with an IC₅₀ value of 3.71±1.09 µM and a Hill coefficient of 0.62±0.14. Although fluvoxamine did not significantly affect the steady-state activation curve, it shifted the steady-state inactivation curve toward a more negative potential. Pretreatment with another SSRI, paroxetine, did not affect the basal Kv current and did not alter the inhibitory effect of fluvoxamine on Kv channels. We concluded that fluvoxamine inhibits the Kv current in a concentration-dependent manner and in a closed (inactivated) state of the Kv channels independent of serotonin reuptake inhibition.

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