Accession Number	<strong>00004630-200111000-00011</strong>.
Author	Kim, D. E. BA *; Phillips, T. M. MS *; Jeng, J. C. MD *+++; Rizzo, A. G. MD ++; Roth, R. T. MD ++; Stanford, J. L. MD ++; Jablonski, K. A. PhD ,[S]; Jordan, M. H. MD *+++
Institution	Bethesda, Maryland, and Washington, DC *Uniformed Services University of the Health Sciences, Bethesda, Maryland; +The Burn Center, Washington, DC; ++Department of Surgery, Washington Hospital Center, Washington, DC; and [S]MedStar Research Institute, Washington, DC
Title	Microvascular Assessment of Burn Depth Conversion during Varying Resuscitation Conditions.[Article]
Source	Journal of Burn Care & Rehabilitation. 22(6):406-416, November/December 2001.
Abstract	Conversion of partial- to full-thickness injuries, even after the burning has stopped, remains a significant clinical problem. We developed a rat model with a wide range of burn depths to study this phenomenon by microvascular assessment. Fifty-four male Sprague-Dawley rats weighing 460 g on average were studied. Real-time tissue monitoring of pH, paCO2, and paO2 was achieved by placement of a continuous blood gas monitor transducer in the aorta. Ten, 2-cm x 2-cm burns were created on each animal with milled aluminum templates (100[degrees]C) with varying contact times. Conversion of burn depth in these wounds was documented by serial laser Doppler imager scanning over a 5-hour period. Animals received Ringer's lactate resuscitation at 0, 2, 4, 6, and 8 ml/kg/%burn. Serial laser Doppler scanning directly demonstrated progressive loss of perfusion to partial-thickness burns dependent upon the amount of fluid resuscitation. Conversion of partial- to full-thickness burns in this rat model (documented by laser Doppler microvascular assessment) was dependent upon how the animals were resuscitated. (C) 2001 Lippincott Williams & Wilkins, Inc.