Hostname: page-component-848d4c4894-m9kch Total loading time: 0 Render date: 2024-06-04T03:17:19.158Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of dietary calcium intake and meal calcium content on calcium absorption in the rat

Published online by Cambridge University Press:  09 March 2007

Kevin D. Cashman  and
Albert Flynn
Kevin D. Cashman
Affiliation:
Department of Nutrition, University College Cork, Republic of Ireland
Albert Flynn
Affiliation:
Department of Nutrition, University College Cork, Republic of Ireland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Fifty-four male and forty-five female 7-week-old rats, Wistar strain, average weights 190 g and 140 g respectively, were randomized by weight into three groups of eighteen rats each (males) and three groups of fifteen rats each (females) and fed on a semi-purified diet containing (per kg) 2 (low), 5 (normal) or 20 g (high) Ca as CaCO3, for 2 weeks. Each group was then further randomized into three groups of six rats each (males) and five rats each (females) and given a meal (10 g of the same diet) containing either 2,5 or 20 g Ca as 47CaCO3. 47Ca was determined in quantitative daily collections of faeces over 7 d and fractional absorption of 47Ca estimated by extrapolating the linear portion (days 3–7) of the plot of log 47Ca retention Y. time back to the time of isotope administration. Absorption of meal Ca was higher in males than in females and was affected similarly in males and females by previous dietary Ca intake and meal Ca content. Fractional absorption of meal Ca decreased with increasing previous dietary Ca intake and with increasing meal Ca content, and the combined effect of these two variables caused fractional Ca absorption to vary from 11–89 %. Absolute absorption of meal Ca decreased with increasing previous dietary Ca intake and increased with increasing meal Ca content. The influence on Ca absorption of variations in meal Ca content (load effect) was greater than that of variations in previous dietary Ca intake (adaptive effect). These results show that previous dietary Ca intake and meal Ca content are both major determinants of Ca absorption from meals in intact rats fed in the normal way and that the rat responds to these factors in a manner similar to that reported for humans. This study provides further evidence of similarities between rats and humans in dietary Ca absorption.

Keywords

Calcium absorptionCalcium intake
Type
General Nutrition
Information
British Journal of Nutrition , Volume 76 , Issue 3 , September 1996 , pp. 463 - 470
Copyright
Copyright © The Nutrition Society 1996

References

REFERENCES

American Institute of Nutrition (1977). Report of the American Institute of Nutrition ad hoc committee on standards for nutritional studies. Journal of Nutrition 107, 1340–1348.CrossRefGoogle Scholar
Benson, J. D., Emery, R. S. & Thomas, J. W. (1969). Effects of previous calcium intakes on adaptation to low and high calcium diets in rats. Journal of Nutrition 97, 53–60.Google Scholar
Brommage, R. (1989). Measurement of calcium and phosphorus fluxes during lactation in the rat. Journal of Nutrition 119, 428–438.Google Scholar
Brommage, R., Baxter, D. C. & Gierke, L. W. (1990). Vitamin D-independent intestinal calcium and phosphorus absorption during reproduction. American Journal of Physiology 259, G631–G638.Google ScholarPubMed
Buchowski, M. S. & Miller, D. D. (1991). Lactose, calcium source and age affect calcium bioavailability in rats. Journal of Nutrition 121, 1746–1754.Google Scholar
Cashman, K. & Flynn, A. (1993). Calcium absorption from cow's milk and soya milks in young rats. Proceedings of the Nutrition Society 52, 273A.Google Scholar
Cross, N. A., Hillman, L. S., Allen, S. H., Krause, G. F. & Vieira, N. E. (1995). Calcium homeostasis and bone metabolism during pregnancy, lactation, and postweaning: a longitudinal study. American Journal of Clinical Nutrition 61, 514–523.Google Scholar
Dostal, L. A. & Toverud, S. U. (1984). Effect of vitamin D3, on duodenal calcium absorption in vivo during early development. American Journal of Physiology 246, G528–G534.Google Scholar
Fairweather-Tait, S. J., Johnson, A., Eagles, J., Ganatra, S., Kennedy, H. & Gurr, M. I. (1989). Studies on calcium absorption from milk using a double-label stable isotope technique. British Journal of Nutrition 62, 379–388.Google Scholar
Hall, B. D., MacMillan, D. R. & Bronner, F. (1969). Vitamin D-resistant rickets associated with high fecal endogenous calcium output: a report of two cases. American Journal of Clinical Nutrition 22, 448–457.CrossRefGoogle Scholar
Heaney, R. P. (1991). Human calcium absorptive performance. In Nutritional Aspects of Osteoporosis. pp. 115–123 [Burckhardt, P. and Heaney, R. P. editors]. New York: Raven Press.Google Scholar
Heaney, R. P., Recker, R. R., Stegman, M. R. & Moy, A. J. (1989). Calcium absorption in women: relationships to calcium intake, estrogen status, and age. Journal of Bone and Mineral Research 4, 469–475.CrossRefGoogle Scholar
Heaney, R. P., Recker, R. R. & Weaver, C. M. (1990). Absorbability of calcium sources: the limited role of solubility. Calcified Tissue International 46, 300–304.CrossRefGoogle ScholarPubMed
Heaney, R. P., Saville, P. D. & Recker, R. R. (1975). Calcium absorption as a function of calcium intake. Journal of Laboratory and Clinical Medicine 85, 881–890.Google ScholarPubMed
Heaney, R. P. & Skillman, T. G. (1971). Calcium metabolism in normal human pregnancy. Journal of Clinical Endocrinology 33, 661–669.CrossRefGoogle ScholarPubMed
Heaney, R. P., Weaver, C. M. & Fitzsimmons, M. C. (1991). Soybean phytate content: effect on calcium absorption. American Journal of Clinical Nutrition 53, 745–747.Google Scholar
Heaney, R. P., Weaver, C. M. & Recker, R. R. (1988). Calcium absorbability from spinach. American Journal of Clinical Nutrition 47, 707–709.CrossRefGoogle ScholarPubMed
Hegsted, M., Schuette, S. A., Zemel, M. B. & Linkswiler, H. M. (1981). Urinary calcium and calcium balance in young men as affected by level of protein and phosphorus intake. Journal of Nutrition 111, 553–562.CrossRefGoogle ScholarPubMed
Henry, K. & Kon, S. K. (1953). The relationship between calcium retention and body stores of calcium in the rat: effect of age and vitamin D. British Journal of Nutrition 7, 147–152.Google Scholar
Ireland, P. & Fordtran, J. S. (1973). Effect of dietary calcium and age on jejunal calcium absorption in humans studied by intestinal perfusion. Journal of Clinical Investigation 52, 2672–2681.Google Scholar
Kaplan, R. A., Haussler, M. R., Deftos, L. J., Bone, H. & Pak, C. Y. C. (1977). The role of 1,25 dihydroxyvitamin D in the mediation of intestinal hyperabsorption of calcium in primary hyperthyroidism and adsorptive hypercalciuria. Journal of Clinical Investigation 59, 756–760.Google Scholar
Lönnerdal, B., Sandberg, A. S., Sandstrom, B. & Kunz, C. (1989). Inhibitory effects of phytic acid and other inositol phosphates on zinc and calcium absorption in suckling rats. Journal of Nutrition 119, 211–214.CrossRefGoogle ScholarPubMed
McCredie, D. A., Troehler, U. & Bonjour, J. P. (1984). In vivo determination of intestinal calcium absorption, with scandium-47 used as a marker. Journal of Laboratory and Clinical Medicine 103, 354–362.Google Scholar
Mahoney, A. W. & Hendricks, D. G. (1978). Some effects of different compounds on iron and calcium absorption. Journal of Food Science 43, 1473–1476.CrossRefGoogle Scholar
Miller, D. D. (1989). Calcium in the diet: food sources, recommended intakes, and nutritional bioavailability. Advances in Food and Nutrition Research 33, 103–156.CrossRefGoogle ScholarPubMed
Miller, S. C., Miller, M. A. & Omura, T. H. (1988). Dietary lactose improves endochondral growth and bone development and mineralization in rats fed a vitamin D-deficient diet. Journal of Nutrition 118, 72–77.CrossRefGoogle ScholarPubMed
Pansu, D., Bellaton, C. & Bronner, F. (1981). Effect of Ca intake on saturable and nonsaturable components of duodenal Ca transport. American Journal of Physiology 240, G32–G37.Google ScholarPubMed
Recker, R. R., Bammi, A., Barger-Lux, M. J. & Heaney, R. P. (1988). Calcium absorbability from milk products, an imitation milk, and calcium carbonate. American Journal of Clinical Nutrition 47, 93–95.CrossRefGoogle ScholarPubMed
Sheikh, M. S., Santa Ana, C. A., Nicar, M. J., Schiller, L. R. & Fordtran, J. S. (1987). Gastrointestinal absorption of calcium from milk and calcium salts. New England Journal of Mediciene 317, 532–536.CrossRefGoogle ScholarPubMed
Snedecor, G. W. & Cochran, W. G. (1967). Statistical Methods. Ames, Iowa: Iowa State University Press.Google Scholar
Tremaine, W. J., Newcomer, A. D., Riggs, B. L. & McGill, B. D. (1986). Calcium absorption from milk in lactase- deficient and lactase-sufficient adults. Digestive Diseases and Sciences 31, 376–378.Google Scholar
Weaver, C. M., Martin, B. R., Ebner, J. S. & Krueger, C. A. (1987). Oxalic acid decreases calcium absorption in rats. Journal of Nutrition 117, 1903–1906.Google Scholar
Weeks, C. E. & King, R. L. (1985). Bioavailability of calcium in heat-processed milk. Journal of Food Science 50, 1101–1105.CrossRefGoogle Scholar
Zemel, M. B. & Linkswiler, H. M. (1981). Calcium metabolism in the young adult male as affected by level and form of phosphorus intake and level of calcium intake. Journal of Nutrition 111, 315–324.CrossRefGoogle Scholar
Ziegler, E. E. & Fomon, S. J. (1983). Lactose enhances mineral absorption in infancy. Journal of Pediatric Gastroenterology and Nutrition 2, 288–294.Google ScholarPubMed