Comparison of Two Rapid Strip Tests Based on IGFBP-1 and PAMG-1 for the Detection of Amniotic Fluid

 

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ABSTRACT

Our objective was to compare two rapid strip tests for the detection of amniotic fluid, based on the detection of insulin-like growth factor-binding protein-1 (IGFBP-1) and of placental α-microglobulin-1 (PAMG-1). Samples of amniotic fluid were taken in 20 pregnant women between 31 3/7 and 41 2/7 gestational weeks at elective cesarean section before delivery of the newborn. These samples were diluted with 0.9% saline solution in a dilution series down to concentrations of 1:320. Immunoassay strip tests were then compared in their ability to detect remaining concentrations of amniotic fluid. In 5 cases, both test methods showed the same results. In all remaining 15 cases, the test based on PAMG-1 proved to be superior by detecting amniotic fluid at least at one descending concentration below the test based on IGFBP-1. Thus, the rapid strip test based on PAMG-1 seems to be a more sensitive bedside test compared with the test based on IGFBP-1 for the detection of amniotic fluid.

REFERENCES

• 1 Lockwood C J, Wein R, Chien D, Ghidini A, Alvarez M, Berkowitz R L. Fetal membrane rupture is associated with the presence of insulin-like growth factor-binding protein-1 in vaginal secretion.  Am J Obstet Gynecol. 1994;  171 146-150
  PubMedGoogle Scholar
• 2 Rutanen E M, Karkkainen T H, Lehtovirta J, Uotila J T, Hinkula M K, Hartikainen A L. Evaluation of a rapid strip test for insulin-like growth factor binding protein-1 in the diagnosis of ruptured fetal membranes.  Clin Chim Acta. 1996;  253 91-101
  CrossrefPubMedGoogle Scholar
• 3 Darj E, Lyrenäs S. Insulin-like growth factor binding protein-1, a quick way to detect amniotic fluid.  Acta Obstet Gynecol Scand. 1998;  77 295-297
  CrossrefPubMedGoogle Scholar
• 4 Guibourdenche J, Luton D, Andre E, Noel M, Porquet D. Rapid detection of insulin-like growth factor-binding protein-1 and foetal fibronectin in cervico-vaginal secretions to diagnose premature membrane rupture.  Ann Clin Biochem. 1999;  36 388-390
  PubMedGoogle Scholar
• 5 Wathen N C, Egembah S, Campbell D J, Farkas A, Chard T. Levels of insulin-like growth factor-binding protein-1 increase rapidly in amniotic fluid from 11 to 16 weeks of pregnancy.  J Endocrinol. 1993;  137 R1-R4
  PubMedGoogle Scholar
• 6 Rutanen E M, Pekonen F, Karkkainen T. Measurement of insulin-like growth factor binding protein-1 in cervical/vaginal secretions: comparison with the ROM-check Membrane Immunoassay in the diagnosis of ruptured fetal membranes.  Clin Chim Acta. 1993;  214 73-81
  CrossrefPubMedGoogle Scholar
• 7 Petrunin D D, Griaznova I M, Petrunina IuA, Tatarinov IuS. Immunochemical identification of organ specific human placental alpha-globulin and its concentration in amniotic fluid.  Akush Ginekol (Mosk). 1977;  (1) 62-64
  PubMedGoogle Scholar
• 8 Cousins L M, Smok D P, Lovett S M, Poeltler D M. AmniSure placental alpha macroglobulin-1 rapid immunoassay versus standard diagnostic methods for detection of rupture of membranes.  Am J Perinatol. 2005;  22 317-320
  Article in Thieme ConnectPubMedGoogle Scholar
• 9 Lee S E, Park J S, Norwitz E R, Kim K W, Park H S, Jun J K. Measurement of placental alpha-microglobulin-1 in cervicovaginal discharge to diagnose rupture of membranes.  Obstet Gynecol. 2007;  109 634-640
  CrossrefPubMedGoogle Scholar

Dr. med. Frank Chih-Kang Chen

Klinik fuer Geburtsmedizin, Charité Universitaetsmedizin Berlin

Campus Virchow-Klinikum, Augustenburger Platz 1,13353 Berlin, Germany