Enzymatic Preparation of Ginsenosides Rg₂, Rh₁, and F₁ from Protopanaxatriol-Type Ginseng Saponin Mixture

 

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Abstract

During investigations on the hydrolysis of a protopanaxatriol-type saponin mixture by various glycoside hydrolases, it was found that two minor saponins, ginsenosides Rg₂ and Rh₁, were formed in high yields by crude β-galactosidase from Aspergillus oryzae and crude lactase from Penicillium sp., respectively. Moreover, a crude preparation of naringinase from Penicillium decumbens readily hydrolyzed a protopanaxatriol-type saponin mixture to give an intestinal bacterial metabolite, ginsenoside F₁ as the main product. A crude preparation of hesperidinase from Penicillium sp. selectively hydrolyzed ginsenoside Re into ginsenoside Rg₁. This is the first report on the enzymatic preparation of minor saponins, ginsenosides Rg₂ and Rh₁, and of an intestinal bacterial metabolite, ginsenoside F₁, with a high efficiency from a protopanaxatriol-type saponin mixture.

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Dr. Sung-Ryong Ko

KT & G Central Research Institute

302 Shinsong-dong

Yusong-ku

Taejon, 305-345, Korea